Immunolocalization of Types V and XI Collagen in Cartilage Using Monoclonal Antibodies
1991; Elsevier BV; Volume: 11; Issue: 2 Linguagem: Inglês
10.1016/s0934-8832(11)80218-0
ISSN2352-0736
AutoresDonald K. Furuto, Renate E. Gay, Tod E. Stewart, Edward J. Miller, Steffen Gay,
Tópico(s)Proteoglycans and glycosaminoglycans research
ResumoMonoclonal antibodies produced against pepsin-solubilized newborn rat skin type V collagen [αl (V)]Zα2(V), and chondrosarcoma type XI collagen [αl (XI)α2(XI)α3(XI)] are used to localize the collagens in sections of the chondrosarcoma as well as the normal rat knee joint by indirect immunofluorescence. Immunostaining for type V collagen shows strong cellular staining of chondrocytes; while the interstitial matrix as well as the lacunae are not stained. In contrast, antitype XI stains not only chondrocytes, but the extracellular compartments as well. In ELISA, rat anti-typeXI collagen reacts with its native antigen, but does not cross-react with native types I, II, III, or V collagen from rat. The distinct locations of type V and XI collagens in cartilaginous tissue suggest varied functional roles for these constituents in the tissue.
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