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CHROMATOGRAPHIC FRACTIONATION OF ORGANIC PHOSPHATES FROM ALKALI, ACID, AND AQUEOUS ACETYLACETONE EXTRACTS OF SOILS

1970; Canadian Science Publishing; Volume: 50; Issue: 2 Linguagem: Inglês

10.4141/cjss70-018

1918-1841

R. L. Halstead, G. ANDERSON,

Plant Micronutrient Interactions and Effects

Extraction with hot 3 N NaOH removed more total organic P, and inositol P fractions, from Scottish and Canadian soils than did extraction with hot 6 N HCl, hot concentrated HCl, or 0.2 M aqueous acetylacetone at pH 8. Only a relatively small amount of the organic P in the acetylacetone extracts could be adsorbed by anion exchange resins unless the extracts were first subjected to hydrolysis with NaOH. This in one case led to a fivefold increase in the phosphate adsorbed and subsequently eluted with NH 4 formate. The proportion of higher inositol phosphates (penta- and hexa-) to the lower esters was greater in the Scottish than the Canadian soils. Paper chromatography revealed that compounds with R f values similar to glycerophosphate and glucose-1-P were present in some fractions. Gas and paper chromatography of the fractions after acid hydrolysis revealed compounds with retention times and R f values corresponding to glycerol and ribitol, but their identity was not confirmed. The neo-, DL-, scyllo- and myo-isomers of the inositol were identified in fractions containing lower inositol phosphates (iP 2 , iP 3 , iP 4 ) and in those containing the higher esters.