Artigo Revisado por pares

Human laminin a chain (LAMA) gene: Chromosomal mapping to locus 18p11.3

1989; Elsevier BV; Volume: 5; Issue: 4 Linguagem: Inglês

10.1016/0888-7543(89)90136-5

ISSN

1089-8646

Autores

T Nagayoshi, Marie‐Geneviève Mattéi, E. Passage, Knowlton Robert, M. L. Chu, Jouni Uitto,

Tópico(s)

Caveolin-1 and cellular processes

Resumo

Laminin, an integral component of basement membranes, consists of three subunit polypeptides, A, B1, and B2 chains. We have recently isolated cDNAs corresponding to human laminin A chain. These cDNAs were utilized for chromosomal in situ hybridizations to establish the genomic location of the laminin A chain gene. Metaphase chromosomes of PHA-stimulated human peripheral blood leukocytes were examined by in situ hybridization with 3H-labeled cDNAs, and the chromosomes were identified by R-banding (fluorochrome-photolysis-Giemsa method). The results indicated that the human laminin A chain is at locus 18p11.3. Since human laminin B1 and B2 chain genes have been previously mapped to chromosomes 7 and 1, respectively, the results indicate that genes encoding human laminin chains reside in separate chromosomes.

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