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Gene Disruption by Homologous Recombination in the Xylella fastidiosa Citrus Variegated Chlorosis Strain

2002; American Society for Microbiology; Volume: 68; Issue: 9 Linguagem: Inglês

10.1128/aem.68.9.4658-4665.2002

1098-5336

Patrice Gaurivaud, Le­onardo Almeida-Souza, A. Virgilio, Anelise Galdino Mariano, Renê R. Palma, Patrícia Brant Monteiro,

Insect symbiosis and bacterial influences

Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.