Monoclonal antibody-based immunoassay of vitellogenin in the blood of male channel catfish (Ictalurus punctatus)
1992; Elsevier BV; Volume: 101; Issue: 3 Linguagem: Inglês
10.1016/0305-0491(92)90025-m
ISSN0305-0491
AutoresAndrew E. Goodwin, John M. Grizzle, James T. Bradley, Barbara H. Estridge,
Tópico(s)Sperm and Testicular Function
Resumo1. A monoclonal antibody to vitellogenin of channel catfish (Ictalurus punctatus) was made, and its specificity was demonstrated using Western blots of serum from female fish, estradiol-treated male fish, untreated male fish, vitellogenin purified by three different methods and egg extracts. 2. An enzyme-linked immunosorbent assay (ELISA), using this monoclonal antibody, detected vitellogenin in the plasma of 59 out of 60 untreated 17–24-month-old male channel catfish with a mean concentration of 338 μg/ml and a maximum concentration of 4240 μg/ml. 3. Vitellogenin levels in male channel catfish were unrelated to testicular stage, gonadosomatic index and month.
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