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SELECTIVE RECOGNITION OF CYCLIC GMP USING A FLUORESCENCE-BASED MOLECULARLY IMPRINTED POLYMER

2002; Taylor & Francis; Volume: 35; Issue: 15 Linguagem: Inglês

10.1081/al-120016540

1532-236X

Nguyễn Thị Kim Thanh, Daniel L. Rathbone, David C. Billington, Nicholas A. Hartell,

Analytical Chemistry and Sensors

ABSTRACT Guanosine 3′,5′-cyclic monophosphate (cGMP) plays a role as a second messenger in many different biological systems. Given the ubiquitous nature of cGMP, a simple method of detecting cGMP is of interest. To that end a fluorescent polymer with recognition sites for cGMP has been prepared. Its selectivity and sensitivity were investigated and a dose-dependant decrease in fluorescence of the polymer in the presence of cGMP was observed. In contrast, virtually no effect was detected upon application of the structurally similar molecules, guanosine 5′-monophosphate (GMP) and adenosine 3′,5′- cyclic monophosphate (cAMP), thus demonstrating the high selectivity of this polymer. The association constant for the binding of cGMP to the imprinted polymer was determined in order of 3 × 105 M−1. A fluorescent, molecularly imprinted polymer that selectively recognises cGMP may have a useful application as a fluorescent chemosensor for cGMP detection in biological samples.