Structural analysis of the specific capsular polysaccharide of Rhodococcus equi serotype
1990; NRC Research Press; Volume: 68; Issue: 4 Linguagem: Inglês
10.1139/o90-112
ISSN1208-6002
AutoresRobert A. Leitch, James C. Richards,
Tópico(s)Polysaccharides and Plant Cell Walls
ResumoThe specific capsular polysaccharide produced by Rhodococcus equi serotype 1 was found to be a high molecular weight acidic polymer composed of D-glucose, D-mannose, and D-glucuronic acid. Structural analysis of the polysaccharide employed a combination of chemical and nuclear magnetic resonance techniques, from which it was determined that the polysaccharide possessed a linear repeating tetrasaccharide unit containing a single O-acetyl substituent and an acetal-linked pyruvic acid moiety:[Formula: see text]The 1 H and 13 C nuclear magnetic resonances of O-deacetylated and pyruvic-free serotype 1 polysaccharides were fully assigned by homo- and hetero-nuclear chemical shift correlation methods.Key words: capsular polysaccharide, structural analysis, Rhodococcus equi.
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