Biosynthesis of 6β-hydroxytestosterone from testosterone by human fetal liver microsomes
1968; Elsevier BV; Volume: 11; Issue: 4 Linguagem: Inglês
10.1016/s0039-128x(68)80068-6
ISSN1878-5867
AutoresJan-Ακε Gustafsson, B. Lisboa,
Tópico(s)Pharmacogenetics and Drug Metabolism
Resumo6β-Hydroxytestosterone has been isolated and characterized after incubation of 4-[14C]-testosterone with 105,000 × g-microsomes of human fetal liver. The presence of 6β-hydroxylase activity in the fetal liver microsomes indicates that the fetal liver — as well as the placenta — is able to form 6β-hydroxy-C19-steroids. The 6β-hydroxytestosterone formed in the fetal liver can be considered as a precursor of the 6-hydroxylated estrogens found in human pregnancy urine. The isolation of this metabolite was carried out by thin-layer chromatographic techniques; the identity of the 6β-hydroxy-testosterone was established by its behaviour on thin-layer chromatography and on gas-liquid chromatography as well as by gas chromatography-mass spectrometry.
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