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Mineralocorticoid receptors in the epithelial cells of human colon and ileum

1984; Pergamon Press; Volume: 20; Issue: 1 Linguagem: Inglês

10.1016/0022-4731(84)90223-1

1878-2353

M.E. Rafestin-Oblin, Marc Lombès, Jean‐Baptiste Michel, Annie Michaud, M. Claire,

Estrogen and related hormone effects

Specific binding of [3H]aldosterone to the cytosolic fraction of epithelial cells was studied in the human colon and terminal ileum. Analysis of [3H]aldosterone binding to the epithelial cells of ascending colon, caecum and ileum as a function of [3H]aldosterone concentration revealed only one class of specific receptors with an affinity constant of about 2 nmol/l. [3H]aldosterone binding was approximately the same in the sigmoid, descending and transverse colon and in the caecum, but slightly lower in the ascending colon and ileum. The specificity of the [3H]aldosterone binding was the same along the colon. The relative order of potency in inhibiting [3H]aldosterone binding was: aldosterone = SC 26304 = dexamethasone much greater than dihydrotestosterone greater than estradiol = RU 26988.