The formation of a ‘pseudo-nerve’ in silicone chambers in the absence of regenerating axons
1992; Elsevier BV; Volume: 592; Issue: 1-2 Linguagem: Inglês
10.1016/0006-8993(92)91664-z
ISSN1872-6240
AutoresQing Zhao, Lars B. Dahlin, Martin Kanje, Göran Lundborg,
Tópico(s)Neuroscience and Neural Engineering
ResumoThe formation of a regenerate between sciatic nerve segments or stumps inserted into Y-tunnelled silicone chambers was studied under conditions where regenerating axons were prevented from entering the chamber. This was accomplished by using an isolated segment of the nerve as a proximal insert. After one week, a cellular regenerate spanned the proximal and distal inserts. The size of the regenerate increased if circulation was preserved in the distal inserts. At four weeks, a perineurium-like sheath surrounded the regenerate and longitudinally oriented Schwann cell columns could be observed throughout the regenerate. A similar ‘pseudo-nerve’ formed towards a piece of distally inserted tendon. Thus, the information required for the formation of a nerve-like structure is inherent to the non-neuronal cells entering the chamber. Schwann cells, in contrast to regenerating axons, do not exhibit preferential growth towards nervous tissue.
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