Ultraviolet Light Induced Connective Tissue Changes in Rat Skin: A Histopathologic and Histochemical Study*
1968; Elsevier BV; Volume: 51; Issue: 4 Linguagem: Inglês
10.1038/jid.1968.123
ISSN1523-1747
AutoresKinuyo Nakamura, Waine C. Johnson,
Tópico(s)Medical and Biological Ozone Research
ResumoIt is gerierally accepted that chroriic exposure to sunlight results in solar elastosis of human skiri.However, experimental production of cutaneous elastotic changes in animals using artificial ultraviolet light has only rarely been reported.Utilizing histochemical methods, Sams, Smith arid Burk demoristrated focal dermal elastosis iii mice following proloriged exposure to artificial ultraviolet light (1).The purpose of this paper is to record our observations ori ultraviolet light iriduced coririective tissue chariges iii rat skiri. MATERIALS AND METHODSThe animals used for this investigation were Fischer strain 344 albino female rats weighing approximately 100 grams at the onset of the experiment.Thirty animals were shaved on the dorsum with electric clippers weekly and exposed to ultraviolet light (UVL) three days a week for a total period of 27 weeks.Fifteen animals were used as controls and were shaved weekly but were not exposed to IJVL.The exposure to UVL was for 20 minutes at a distance of 62 centimeters from a bank of 4 Westinghouse FS 40 fluorescent sun lamps.Biopsies from the dorsum of 4 treated animals and 2 control animals were taken after one week of TJVL exposure and subsequently at 2 week intervals throughout the 27 week period of light exposure and afterward for a total period of 46 weeks.At 46 weeks, all of the IJVL treated animals and all control animals were sacrificed for additional histopathologic and histochemical studies.One-half of each tissue specimen was frozen on solid carbon dioxide and the remainder was embedded in paraffin.Fifty microns cryostat sections were processed for demonstration of blood vessels using the alkaline phosphatase technique
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