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Inhibition of DNA polymerase-α and -β of calf thymus by 1-β-d-arabinofuranosylcytosine-5′-triphosphate

1977; Elsevier BV; Volume: 477; Issue: 2 Linguagem: Inglês

10.1016/0005-2787(77)90230-1

1879-3002

Shonen Yoshida, Machiko Yamada, Shigeo Masaki,

Biochemical and Molecular Research

1-beta-D-Arabinofuranosylcytosine 5'-triphosphate (araCTP), an active form of a inhibitor of DNA replication, 1-beta-D-arabinofuranosylcytosine (araC) was tested for its inhibitory action on the DNA polymerase-alpha and -beta (EC 2.7.7.7) purified from calf thymus. The reaction of DNA polymerase-alpha was shown to be more sensitive to the inhibition by araCTP than that of DNA polymerase-beta. The mode of the inhibition by araCTP was competitive to dCTP in the reaction catalysed by either DNA polymerase-alpha or -beta. The Ki value of DNA polymerase-beta for araCTP was 32 micron; eight times higher than that of DNA polymerase-alpha (4 micron) for this inhibition.