A semiautomatic image analyzer for cell counts in monolayers. I. Construction, experimental procedure, and precision obtained
1989; Taylor & Francis; Volume: 27; Issue: 4 Linguagem: Inglês
10.1080/15287398909531319
ISSN1087-2620
Autores Tópico(s)Computational Drug Discovery Methods
ResumoA simple, flexible, low‐cost image analyzer system is presented for determination of the total number of cells and the number of viable cells in monolayers of nonconfluent cells. Viable cells were identified and counted using an immunofluorescence method using fluorescein diacetate. Alveolar macrophages from rabbits were used. The different possible counting procedures that could be performed are analyzed. The precision of the method of estimating the cell numbers is indicated by a coefficient of variation of 5%. The experimental procedure is given for a short‐term toxicological in vitro test in which the semiautomatic counting procedures can be performed within 1.5 h.
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