The relationship between the cholesterol content and subfraction distribution of plasma high-density lipoproteins
1980; Elsevier BV; Volume: 101; Issue: 1 Linguagem: Inglês
10.1016/0009-8981(80)90055-8
ISSN1873-3492
AutoresJames Shepherd, Christopher J. Packard, Jennifer M. Stewart, B D Vallance, T. D. V. Lawrie, Hugh Morgan,
Tópico(s)Lipid metabolism and disorders
ResumoHigh density lipoprotein subfractions (HDL2 and HDL3) were separated from the plasma of 25 healthy volunteers (13 males, 12 females) by rate zonal ultra-centrifugation. The rotor elution profile, measured at 280 nm, was used with the specific extinction coefficient for each subfraction (HDL2, 0.60 ± 0.11 mg protein/A280nm, HDL3, 0.86 ± 0.10 mg protein/A280nm(n = 25) to calculate their plasma concentration. Their protein and lipid composition were also determined by chemical analysis. Plasma lipids, lipoprotein cholesterol, apolipoprotein A-I, apolipoprotein A-II and apolipoprotein B levels were measured in the same subjects and correlated with the HDL subfraction concentrations. HDL cholesterol and apolipo-protein A-I concentrations correlated significantly (p< 0.01 and 0.02 respectively) with plasma HDL2, but not with HDL3. Indeed, the significantly higher levels of HDL cholesterol and apolipoprotein A-I in the female group could be attributed entirely to an increase in circulating HDL2. This data supports the proposal that the latter subfraction is the major contributor to the anti-atherogenic role of plasma HDL.
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