Use of sulphopropyl-sephadex chromatography and disc gel electrophoresis for the fractionation and identification of Elapid venoms
1974; Elsevier BV; Volume: 12; Issue: 4 Linguagem: Inglês
10.1016/0041-0101(74)90008-7
ISSN1879-3150
AutoresVirginia B. Chatman, Samuel J. DiMari,
Tópico(s)Cellular transport and secretion
ResumoAbstract The cation exchange resin Sulphopropyl (SP)-Sephadex has been used for the preparative isolation of both neurotoxins and cytotoxins from various Elapid venoms. The chromatographic system described affords high resolution of venom components upon elution with simple linear salt gradients. Cytotoxins from the venom of Naja naja naja (India) and neurotoxins from Naja naja atra (Formosa), Naja naja siamensis and Bungarus multicinctus have been isolated in high yield as pure, physiologically-active materials. The resin has also been applied, together with two different discontinuous gel electrophoresis systems, to the characterization of venoms from ten Asian Naja naja subspecies. All of the venoms studied could be identified by their disc gel acidic and basic protein patterns alone, or by a combination of their SP-Sephadex protein elution profiles and the disc gel patterns of their acidic proteins. These studies indicate that the systems reported could be used not only for the identification of specific venoms but could also serve as a basis for a classification system for snake venoms.
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