Produção Nacional
Revisado por pares
Optimization of Extraction of Lipase from Wheat Seeds (Triticum aestivum) by Response Surface Methodology
2009; American Chemical Society; Volume: 57; Issue: 20 Linguagem: Inglês
10.1021/jf901816x
ISSN1520-5118
AutoresMorgana Karin Pierozan, Roger Junges da Costa, O. A. C. Antunes, Enrique G. Oestreicher, J. Vladimir Oliveira, Rogério Luís Cansian, Helen Treichel, Débora de Olíveira,
Tópico(s)Agricultural and Food Sciences
ResumoThis work aimed to evaluate the effects of particle size, solid/solvent ratio (w/v), and reaction time on hydrolytic and esterification activities of a lipase extract from wheat seeds. The higher hydrolytic activity was 5.9 U/g with a particle size of 425 microm, solid/solvent ratio of 30:60 (w/v), and reaction time of 15 h, with maximum hydrolytic activity of 14.47 U/g after 24 h of precipitation. For esterification activity the best result was 57.88 U/g with a particle size of 425 microm and solid/solvent ratio of 30:120 (w/v) for 5 h, with a maximum value after 10 h of precipitation reaching 208.20 U/g. The partial characterization showed that the optimal pH and temperature were found to be 5.5 and 32-37 degrees C, respectively. The extract stability at low temperatures was kept after 48 h of storage in terms of esterification activity. The hydrolytic activity was kept constant at -10 degrees C during 72 h and diminished considerably after 24 h at 4 degrees C.
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