“Effect of apoüpoprotein E4 alíele on plasma LDLcholesterol response to diet therapy in type 2 diabetic patients”
2004; Generalitat of Catalonia; Volume: 39; Issue: 144 Linguagem: Inglês
10.1016/s1886-6581(04)76079-x
ISSN1886-6581
AutoresMiyuki Saito, Masato Eto, Hayami Nitta, Y Kanda, Makoto Shigeto, Keizo Nakayama, Kazuhito Tawaramoto, Fumiko Kawasaki, Shinji Kamei, Kenji Kohara, M Matsuda, Michihiro Matsuki, Kohei Kaku, Heather K. Vincent, James W. Morgan, Kevin R. Vincent, Eray Copçu,
Tópico(s)Diabetes, Cardiovascular Risks, and Lipoproteins
ResumoIn this work we have studied the complexation of curcumin (HCur, 1,7-bis-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione) with Al(III) and Fe(III) at 37 °C and in 0.16 M NaCl. The protonation constant of curcumin was also determined to evaluate the competition of the ligand for the metal cations and H+. Speciation profiles obtained by potentiometric titrations show that in aqueous solution a complexation occurs at 1:1 ligand-to-cations ratio. With the aim to define the coordination sites of curcumin with these metal ions a mass spectrometry and UV–Vis spectroscopy characterization was carried out. It was found that aluminum binds to the keto-enol moiety of curcumin, while iron binds to the guaiacol (2-methoxyphenol) one. This unexpected experimental evidence was supported by a thorough computational study.
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