Antibiotic monitoring in body fluids
1985; Elsevier BV; Volume: 340; Linguagem: Inglês
10.1016/0378-4347(85)80202-4
ISSN1872-812X
Autores Tópico(s)Analytical Chemistry and Chromatography
ResumoAnalytical procedures recently described for the quantitative determination of antibiotics in body fluids are reviewed. High-performance liquid chromatography (HPLC) and immunoassays appear as an alternative to current microbiological assays. HPLC has been applied to most antibiotics in clinical use and a major part of the review deals with this technique. Attention is given to sample pretreatment, characteristics of chromatography and detection, and limit of sensitivity. Non-isotopic immunoassays have been essentially applied to aminoglycosides and vancomycin and are also reviewed. Advantages and drawbacks of HPLC and immunoassays are presented.
Referência(s)