Immunological studies of γδ t cells in a case of large granular lymphocyte (LGL) leukemia: Leukemic γδ+ T cells are resistant to growth stimulation in vitro but respond to interferon-α treatment in vivo
1992; Elsevier BV; Volume: 16; Issue: 11 Linguagem: Inglês
10.1016/0145-2126(92)90047-b
ISSN1873-5835
AutoresRalf Metzger, Brigitte Heckl‐Östreicher, Christoph Nerl, S Schondelmaier, Dieter Kabelitz,
Tópico(s)Immunodeficiency and Autoimmune Disorders
ResumoLeukemic T cells from patient BU (WBC 22000/1) resembled morphologically large granular lymphocytes, and expressed a Vδ1-encoded γδ T-cell receptor (TCR) an their surface. Upon in vitro activation with various mitogens, IL-2-dependent Vδ1+ cell lines were only obtained if Vδ1+ cells had been positively selected an a cell sorter before culture. However, even under these conditions, the Vδ11 IL-2-dependent Gell lines showed a TCR β and γ gene-rearrangement different from that of the freshly isolated Leukemic cell population, indicating that they were not derived from the Leukemic clone. Thus, known T-cell growth factors (IL-2, IL-4, IL-7) in concert with mitogenic signals failed to induce in vitro proliferation of the Vδ1+ Leukemic clone. Interestingly, the patient responded to treatment with interferon-α2c (daily dose of 106 I.U.). After 212 years of continuous interferon treatment, the patient is in partial remission with WBC around 8000/μml.
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