Application of contour maps to three-dimensional display of ultraviolet absorbance in high-performance liquid chromatography of natural drug materials
1986; Elsevier BV; Volume: 362; Linguagem: Inglês
10.1016/s0021-9673(01)86960-0
ISSN1873-3778
AutoresKiichiro Kajiyama, Yohei Ohno, Toshiro Ochiai, Yukio Hiraga, Kunio Takahashi,
Tópico(s)Phytochemicals and Antioxidant Activities
ResumoThe use of reversed-phase high-performance liquid chromatography for the determination of the biologically active plant phenolic compounds mangiferin, likviritin and dihydroquercetin is described. Perchloric acid (35%) was used for deproteinization in the case of mangiferin and likviritin, and acidified methanol for dihydroquercetin. Detection was performed at 254, 275 and 290 nm for mangiferin, likviritin and dihydroquercetin in plasma, and 365, 312 and 290 nm in urine, respectively. The limit of detection was 0.2 μg/ml for plasma and 0.5 μg/ml for urine.
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