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Fluorescent pigments from uterus of vitamin E‐deficient rats

1975; Wiley; Volume: 10; Issue: 5 Linguagem: Inglês

10.1007/bf02532706

1558-9307

I. D. Desai, Brenda L. Fletcher, Al L. Tappel,

Free Radicals and Antioxidants

Abstract Spectrophotofluorometric analyses of the extracts of uterus from sterile rats maintained on a vitamin E‐deficient diet for a prolonged period of time indicated that the brown ceroid‐like pigments present were highly fluorescent, having characteristics typical of products formed during lipid peroxidation in vivo. The aqueous‐methanol layer of the uteral extract from vitamin E‐deficient animals had higher fluorescence than the corresponding chloroform layer, indicating the presence of more polar water‐soluble pigments than those found in most other tissues so far examined.