Chemoenzymatic one-pot synthesis of cefazolin from cephalosporin C in fully aqueous medium, involving three consecutive biotransformations catalyzed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase
1997; Elsevier BV; Volume: 38; Issue: 26 Linguagem: Inglês
10.1016/s0040-4039(97)00999-4
ISSN1873-3581
AutoresRoberto Fernandéz‐Lafuente, JoséM. Guisán, Massimo Pregnolato, Marco Terreni,
Tópico(s)Synthesis and Catalytic Reactions
ResumoA new chemoenzymatic synthesis of Cefazolin through the correct assembly of three biotransformations catalysed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase is described. This multienzymatic synthesis has been performed from the natural Cephalosporin C in fully aqueous medium without intermediate purification stages. Almost quantitative yields have been achieved in all the enzymatic reactions.
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