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BIBTEX RIS

Effect of Addition of Orvus ES Paste to Frozen Canine Semen Extender on Sperm Acrosomes.

2000; Japanese Society of Veterinary Science; Volume: 62; Issue: 5 Linguagem: Inglês

10.1292/jvms.62.537

ISSN

1347-7439

Autores

Toshihiko TSUTSUI, Masayoshi HASE, Tatsuya HORI, Kaoru KOMORIYA, Noriko Shimizu, Keiichi NAGAKUBO, Eiichi KAWAKAMI,

Tópico(s)

Animal Genetics and Reproduction

Resumo

Using the triple-stain technique, we investigated whether sperm acrosomes in frozen canine semen were protected during freezing and thawing by addition of a surfactant, Orvus ES Paste (OEP), to the extender. Acrosomes were clearly shown to be protected by the addition of OEP to the entender when compared with those in sperm frozen without OEP addition (p<0.05).

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