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T lineage differentiation from human embryonic stem cells

2006; National Academy of Sciences; Volume: 103; Issue: 31 Linguagem: Inglês

10.1073/pnas.0604244103

1091-6490

Zoran Galić, Scott G. Kitchen, Amelia Kacena, Aparna Subramanian, Bryan Burke, Ruth Cortado, Jerome A. Zack,

CAR-T cell therapy research

Harnessing the ability of genetically manipulated human embryonic stem cells (hESC) to differentiate into appropriate lineages could revolutionize medical practice. These cells have the theoretical potential to develop into all mature cell types; however, the actual ability to develop into all hematopoietic lineages has not been demonstrated. Using sequential in vitro coculture on murine bone marrow stromal cells, and engraftment into human thymic tissues in immunodeficient mice, we demonstrate that hESC can differentiate through the T lymphoid lineage. Stable transgene expression was maintained at high levels throughout differentiation, suggesting that genetically manipulated hESC hold potential to treat several T cell disorders.