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Penicillium mallochii and P. guanacastense , two new species isolated from Costa Rican caterpillars

2012; Mycotaxon Publications; Volume: 119; Issue: 1 Linguagem: Inglês

10.5248/119.315

2154-8889

Karol Geraldina Rivera, Joel Díaz, Felipe Chavarría-Díaz, M.L. Garcı́a, Mirjam Urb, R. Greg Thorn, Gerry Louis-Seize, Daniel H. Janzen, Keith A. Seifert,

Fungal Biology and Applications

Twenty-five strains of monoverticillate Penicillium species were isolated from dissected guts and fecal pellets of leaf-eating caterpillars reared in the Área de Conservación Guanacaste, Costa Rica, or from washed leaves of their food plants. Phylogenetic analyses of β-tubulin, nuclear ribosomal internal transcribed spacer (ITS), cytochrome c oxidase subunit 1, translation elongation factor 1- α and calmodulin gene sequences revealed two phylogenetically distinct, undescribed species closely related to P. sclerotiorum . Penicillium mallochii was isolated from Rothschildia lebeau and Citheronia lobesis ( Saturniidae ) and their food plant Spondias mombin ( Anacardiaceae ) and P. guanacastense from Eutelia sp. ( Noctuidae ). Both fungi produce greenish conidial masses and orange pigments in agar culture, have smooth-walled, monoverticillate conidiophores with moderately vesiculate apices, and globose to subglobose conidia. The species morphologically resemble P. sclerotiorum but differ subtly in vesicle width and conidial shape.