Nephronophthisis type 1 deletion syndrome with neurological symptoms: Prevalence and significance of the association
2006; Elsevier BV; Volume: 70; Issue: 7 Linguagem: Inglês
10.1038/sj.ki.5001768
ISSN1523-1755
AutoresGianluca Caridi, Monica Dagnino, Andrea Rossi, Enza Maria Valente, Enrico Bertini, Elisa Fazzi, Francesco Emma, Luisa Murer, Enrico Verrina, Gian Marco Ghiggeri,
Tópico(s)Renal and related cancers
ResumoType 1 nephronophthisis (NPHP) with homozygous deletions of nephrocystin [NPHP1, DEL] has been considered a pure renal disorder, but co-occurrence of extrarenal symptoms, mainly retinitis pigmentosa, is observed in a subset of patients. Recently, [NPHP1, DEL] has been detected in three patients with Joubert syndrome-related disorders (JSRDs), who associated neurological signs with a peculiar neuroradiological malformation known as the ‘molar tooth sign’ (MTS). To define the frequency of JSRD spectrum in NPHP1 patients, we re-examined 56 cases with [NPHP1, DEL] and found an overall incidence of 8.9% (five out 56 patients). All had small hyperechoic kidneys and had developed advanced renal failure within 15 years. Two patients presented the complete features of JSRD with cerebello-renal-retinal association and MTS. Two others showed, instead, severe intentional tremor and thick superior cerebellar peduncles on brain magnetic resonance imaging (MRI), and one of them had associated retinopathy. The fifth patient presented with hypotonia, developmental delay, central deafness, and ataxia associated with Leber congenital amaurosis and liver fibrosis but with normal brain MRI. Marked intrafamilial variability of associated extrarenal symptoms was observed in familial cases. Deletion extension did not differ in patients with isolated renal phenotype and in those with associated neurological symptoms. In conclusion, neurological defects varying from subtle involvement of cerebellum with thickened peduncle to both JSRD and diffuse central hypotonia are frequent in [NPHP1, DEL] patients. Prevalence of such association may justify systematic neurological and neuroradiological evaluation. Type 1 nephronophthisis (NPHP) with homozygous deletions of nephrocystin [NPHP1, DEL] has been considered a pure renal disorder, but co-occurrence of extrarenal symptoms, mainly retinitis pigmentosa, is observed in a subset of patients. Recently, [NPHP1, DEL] has been detected in three patients with Joubert syndrome-related disorders (JSRDs), who associated neurological signs with a peculiar neuroradiological malformation known as the ‘molar tooth sign’ (MTS). To define the frequency of JSRD spectrum in NPHP1 patients, we re-examined 56 cases with [NPHP1, DEL] and found an overall incidence of 8.9% (five out 56 patients). All had small hyperechoic kidneys and had developed advanced renal failure within 15 years. Two patients presented the complete features of JSRD with cerebello-renal-retinal association and MTS. Two others showed, instead, severe intentional tremor and thick superior cerebellar peduncles on brain magnetic resonance imaging (MRI), and one of them had associated retinopathy. The fifth patient presented with hypotonia, developmental delay, central deafness, and ataxia associated with Leber congenital amaurosis and liver fibrosis but with normal brain MRI. Marked intrafamilial variability of associated extrarenal symptoms was observed in familial cases. Deletion extension did not differ in patients with isolated renal phenotype and in those with associated neurological symptoms. In conclusion, neurological defects varying from subtle involvement of cerebellum with thickened peduncle to both JSRD and diffuse central hypotonia are frequent in [NPHP1, DEL] patients. Prevalence of such association may justify systematic neurological and neuroradiological evaluation. Nephronophthisis (NPHP) is an autosomal-recessive renal disorder with onset in infancy or childhood, representing the most frequent genetic cause of end-stage renal failure (ESRF) in children. NPHP is clinically heterogeneous, with a subset of patients presenting associated extrarenal defects. Six causative genes have been now identified (NPHP1–6).1.Hildebrandt F. Otto E. Rensing C. et al.A novel gene encoding an SH3 domain protein is mutated in nephronophthisis type 1.Nat Genet. 1997; 17: 149-153Crossref PubMed Scopus (259) Google Scholar, 2.Otto E.A. Schermer B. Obara T. et al.Mutations in INVS encoding inversin cause nephronophthisis type 2, linking renal cystic disease to the function of primary cilia and left–right axis determination.Nat Genet. 2003; 34: 413-420Crossref PubMed Scopus (476) Google Scholar, 3.Olbrich H. Fliegauf M. Hoefele J. et al.Mutations in a novel gene NPHP3, cause adolescent nephronophthisis, tapeto-retinal degeneration and hepatic fibrosis.Nat Genet. 2003; 34: 455-459Crossref PubMed Scopus (271) Google Scholar, 4.Mollet G. Salomon R. Gribouval O. et al.The gene mutated in juvenile nephronophthisis type 4 encodes a novel protein that interacts with nephrocystin.Nat Genet. 2002; 32: 300-305Crossref PubMed Scopus (171) Google Scholar, 5.Otto E.A. Loeys B. Khanna H. et al.Nephrocystin-5, a ciliary IQ domain protein, is mutated in Senior–Loken syndrome and interacts with RPGR and calmodulin.Nat Genet. 2005; 37: 282-288Crossref PubMed Scopus (282) Google Scholar, 6.Valente E.M. Silhavy J.L. Brancati F. et al.Mutations in CEP290, which encodes a centrosomal protein, cause pleiotropic forms of Joubert syndrome.Nat Genet. 2006; 38: 623-625Crossref PubMed Scopus (283) Google Scholar, 7.Sayer J.A. Otto E.A. O'Toole J.F. et al.The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4.Nat Genet. 2006; 38: 674-681Crossref PubMed Scopus (395) Google Scholar Homozygous deletions of the NPHP1 gene at 2q13 [NPHP1, DEL] are responsible for about 40% of cases8.Konrad M. Saunier S. Heidet L. et al.Large homozygous deletions of the 2q13 region are a major cause of juvenile nephronophthisis.Hum Mol Genet. 1996; 5: 367-371Crossref PubMed Scopus (122) Google Scholar, 9.Caridi G. Dagnino M. Miglietti N. et al.Juvenile nephronophthisis and related variants: clinical features and molecular approach.Contrib Nephrol. 2001; 136: 57-67Crossref PubMed Scopus (2) Google Scholar, 10.Hildebrandt F. Rensing C. Betz R. et al.Establishing an algorithm for molecular genetic diagnostics in 127 families with juvenile nephronophthisis.Kidney Int. 2001; 59: 434-445Abstract Full Text Full Text PDF PubMed Scopus (33) Google Scholar and usually give rise to a pure renal phenotype. However, association with pigmentary retinopathy (Senior–Loken syndrome) or oculomotor apraxia has been reported in NPHP1 patients.11.Caridi G. Murer L. Bellantuono R. et al.Renal–retinal syndromes: association of retinal anomalies and recessive nephronophthisis in patients with homozygous deletion of the NPH1 locus.Am J Kidney Dis. 1998; 32: 1059-1062Abstract Full Text PDF PubMed Scopus (62) Google Scholar,12.Betz R. Rensing C. Otto E. et al.Children with ocular motor apraxia type Cogan carry deletions in the gene (NPHP1) for juvenile nephronophthisis.J Pediatr. 2000; 136: 828-831Abstract Full Text PDF PubMed Scopus (77) Google Scholar Recently, [NPHP1, DEL] was identified in three patients with Joubert syndrome-related disorders (JSRDs), a group of syndromes showing the typical neurological signs of Joubert syndrome (hypotonia, ataxia, psychomotor delay, and oculomotor apraxia-type Cogan) and variable involvement of other organs such as the eye and kidneys.13.Parisi M.A. Bennett C.L. Eckert M.L. et al.The NPHP1 gene deletion associated with juvenile nephronophthisis is present in a subset of individuals with Joubert syndrome.Am J Hum Genet. 2004; 75: 82-91Abstract Full Text Full Text PDF PubMed Scopus (186) Google Scholar,14.Castori M. Valente E.M. Donati M.A. et al.NPHP1 gene deletion is a rare cause of Joubert syndrome related disorders.J Med Genet. 2005; 42: e9Crossref PubMed Scopus (71) Google Scholar All JSRDs share a pathognomonic malformation of the midbrain–hindbrain junction known as the ‘molar tooth sign’ (MTS), consisting of cerebellar vermis hypo/aplasia, thick and mal-oriented superior cerebellar peduncles, and abnormally deep interpeduncular fossa.15.Louie C.M. Gleeson J.G. Genetic basis of Joubert syndrome and related disorders of cerebellar development.Hum Mol Genet. 2005; 14: R235-R242Crossref PubMed Scopus (67) Google Scholar JSRDs are genetically heterogeneous, with two genes (JBTS3 – AHI1 at 6q23.3 and JBTS5 – CEP290 at 12q21.32) and two additional loci (JBTS1 at 9q34.3 and JBTS2 at 11p11.2–q12.3) identified so far.16.Valente E.M. Marsh S.E. Castori M. et al.Distinguishing the four genetic causes of Jouberts syndrome-related disorders.Ann Neurol. 2005; 57: 513-519Crossref PubMed Scopus (81) Google Scholar Mutations in the AHI1 gene are occasionally responsible for JSRDs with renal/retinal involvement; at this time, only three families with affected sibling and NPHP are described.17.Utsch B. Sayer J.A. Attanasio M. et al.Identification of the first AHI1 gene mutations in nephronophthisis-associated Joubert syndrome.Pediatr Nephrol. 2006; 21: 32-35Crossref PubMed Scopus (66) Google Scholar,18.Parisi M.A. Doherty D. Eckert M.L. et al.AHI1 mutations cause both retinal dystrophy and renal cystic disease in Joubert syndrome.J Med Genet. 2006; 43: 334-339Crossref PubMed Scopus (89) Google Scholar Recently, a second gene for Joubert syndrome (CEP290) was identified by two independent groups6.Valente E.M. Silhavy J.L. Brancati F. et al.Mutations in CEP290, which encodes a centrosomal protein, cause pleiotropic forms of Joubert syndrome.Nat Genet. 2006; 38: 623-625Crossref PubMed Scopus (283) Google Scholar,7.Sayer J.A. Otto E.A. O'Toole J.F. et al.The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4.Nat Genet. 2006; 38: 674-681Crossref PubMed Scopus (395) Google Scholar in a series of families presenting both neurological and renal symptoms. These observations suggest overlapping phenotypes to those described in NPHP1-deleted patients. To evaluate the frequency and clinical spectrum of neurological signs associated with NPHP1, we have re-evaluated 56 patients with [NPHP1, DEL] and performed detailed clinical and neuroradiological examination in those with possible neurological involvement. Among the 125 patients with NPHP, 56 patients presented [NPHP1, DEL] and four cases carried NPHP1 mutations. This study focuses on the wide clinical aspects of only the subset of patients with [NPHP1, DEL]. The remaining 65 patients without NPHP1 deletion/mutation and the four patients presenting NPHP1 mutations19.Caridi G. Dagnino M. Trivelli A. et al.Stop codon at arginine 586 is the prevalent nephronophthisis type 1 mutation in Italy.Nephrol Dial Transplant. 2006https://doi.org/10.1093/ndt/gfl277Google Scholar had no signs of neurological involvement and were not included in the study cohort. All patients with [NPHP1, DEL] presented typical renal symptoms, including polyuria, polydipsia, low urine osmolality, and small kidneys without corticomedullary differentiation and occasional cysts. Creatinine levels were variable and independent from age, whereas renal failure was a constant finding. Five patients (8.9%) had signs of neurological involvement, and underwent detailed neurological and ophthalmologic examination and brain magnetic resonance imaging (MRI). Clinical features of these five subjects are presented in Table 1. Three patients had at least another sibling with [NPHP1, DEL] (Fam.2, ZR; Fam.3, CM; and Fam.4, PC), whereas two cases (Fam.1, PL and Fam.5, LF) were sporadic. In familial cases, neurological and ophthalmologic assessment was extended to the available affected siblings even in the absence of obvious neurological symptoms.Table 1Clinical details of the five families with homozygous NPHP1 deletion associated with neurological defectsFam.1Fam.2Fam.3Fam.4Fam.5Patient (sex)PL (M)ZR (M)ZS (F)CC (M)CM (M)CD (M)PC (M)PE (F)LF (M)Age (years)113141211814363215Kidneys Polyuria/polydipsia+++++++++++ Urine osm (mOsm/l)<250<250<250<250<250<250<250<250<250 Creatinine (mg%)−−−1,3054−−2 End-stage renal failurePDHD/TXHD/TX−−−HD/TXHD/TX−Central nervous system Psychomotor delay++−−−−−−− Intentional tremor++−−+++++−+ Ataxia++−−−−−−+ Hypotonia++−−−−−−+ Oculomotor apraxia++−−−−−−− Nystagmus++−−−−−−+Brain MRI Molar tooth sign++ND−−−−ND− Thick superior cerebellar peduncles−−ND−+−+ND−Eyes LCA/retinitis pigmentosa−++−−−+−+ Flat electroretinogramND+ND−−−+−+Liver Elevated enzymes−−−−−−−−+ Abnormal ultrasound−−−−−−−−+Other signs−−−−−−−−DeafnessHD, hemodialysis; LCA, Leber congenital amaurosis; MRI, magnetic resonance imaging; ND, not determined; NPHP, nephronophthisis; PD, peritoneal dialysis; TX, renal transplant. Open table in a new tab HD, hemodialysis; LCA, Leber congenital amaurosis; MRI, magnetic resonance imaging; ND, not determined; NPHP, nephronophthisis; PD, peritoneal dialysis; TX, renal transplant. Two patients from two different families (Fam.1, PL and Fam.2, ZR) received a diagnosis of JSRD based on the neuroradiological finding of the MTS (Figure 1). Patient 1 (Fam.1, P.L) is a 12-year-old boy who developed ESRF and required peritoneal dialysis at the age of 11 years. Oculomotor apraxia, horizontal nystagmus, ataxia with severe walking difficulties, and moderate tremor had been present for years, but had not been further investigated until the present study. No retinal symptoms were present. Brain MRI demonstrated a mild MTS with moderate inferior vermis hypoplasia, narrowing of the isthmus, and superior cerebellar peduncles that were maloriented but not thickened (Figure 1). Patient 2 (Fam.2, ZR) had developed ESRF at the age of 15 years. He had a renal transplant at the age of 20 years and died of infectious complications at 31 years. Clinical records reported early psychomotor delay and hypotonia. Fundus oculi examination had demonstrated retinitis pigmentosa and electroretinogram was abnormal.11.Caridi G. Murer L. Bellantuono R. et al.Renal–retinal syndromes: association of retinal anomalies and recessive nephronophthisis in patients with homozygous deletion of the NPH1 locus.Am J Kidney Dis. 1998; 32: 1059-1062Abstract Full Text PDF PubMed Scopus (62) Google Scholar A brain MRI, performed at the age of 17 years, showed the MTS with vermis hypoplasia, narrowing of the isthmus, and elongation of superior cerebellar peduncles (Figure 1). Two siblings of the proband carried the [NPHP1, DEL]. His sister, aged 41 years (Fam.2 ZS), developed ESRF at 17 years, which required hemodialysis and renal transplant. She denied any neurological symptoms and refused to undergo further diagnostic assessment. The proband's brother died of renal failure at 10 years; no further clinical details are available. The association of intense intentional tremor with thickened superior cerebellar peduncles on brain MRI (in the absence of a clear MTS) was observed in two patients from two distinct families (Figure 2; Fam.3, CM and Fam.4, PC). Family 3 comprises three male patients of 14, 18, and 21 years who carry the [NPHP1, DEL]. The two younger brothers had ESRF with creatinine serum levels of 4 and 5 mg%, respectively, whereas creatinine was only slightly elevated (1.3 mg%) in the oldest brother. The proband presented from the age of 10 years an intense action and postural tremor upon extending arms and/or performing common tasks such as writing or eating, which severely impaired daily life activities. Video polygraphy was altered according to clinical symptoms, whereas retinal examination was unremarkable. A brain MRI, performed at the age of 17 years, showed normal cerebellar vermis but thickening of superior cerebellar peduncles (Figure 2; Fam.3, CM). The proband's younger brother (Fam.3, CD) had mild intentional arm tremor, whereas the elder brother (Fam3, CC) was neurologically normal. Both sibling had a normal brain MRI scan. Patient 4 is a 36-year-old man who developed ESRF at the age of 10 years (Figure 2; Fam.4, PC). He received two renal grafts: the first was lost for acute rejection, whereas the second was still functioning after 3 years. He reported intense intentional arm tremor from the age of 5 years with severe impairment of most daily living activities. Video polygraphy was markedly altered. The legs were not affected and he could walk normally. Brain MRI showed normal cerebellar vermis and thickened superior cerebellar peduncles (Figure 1; case 4). The patient who reported nocturnal visual impairment and visual assessment at the age of 15 years showed a flat electroretinogram. The proband's sister (Figure 2; Fam.4, PE), aged 32 years, also carries [NPHP1, DEL] and developed ESRF at 13 years. Her neurological and ophthalmologic examination were normal and she refused to undergo brain MRI. Patient 5 (Fam.5, LF), aged 15 years, presented mild developmental delay, hypotonia, and reduced hearing function from birth. Visual loss was evident at the age of 2 years and the patient became blind by the age of 10 years. Ophthalmologic examination showed patchy retinal deposits of pigments and a flat electroretinogram, whereas audiometry- and acoustic-evoked potentials demonstrated central hearing loss. On latest examination, he presented mild hypotonia and fine tremor of fingers upon arm extension, with no other neurological abnormalities and without mental retardation. Brain MRI was unremarkable. Liver enzymes were constantly elevated and an abdominal ultrasound showed liver hyperechogenicity, suggesting hepatic fibrosis. A sister of 9 years is healthy. The extension of the deleted region was evaluated in all [NPHP1, DEL] patients according to the scheme described in Materials and Methods. Fifty-four patients (including patients with JSRD) carried the classical ∼250 kb homozygous deletion from exon 2 of LOC205251 to 9657T markers, located in intron 1 of MALL. Two patients with a pure renal form of NPHP1 carried ‘instead’ a shorter (∼180 kb) deletion spanning from 804/6 to 9657T (Table 2).Table 2List of molecular STS markers utilized for testing the presence of NPHP1 deletion Open table in a new tab Neurological symptoms can be part of the clinical spectrum of [NPHP1, DEL], but a real estimate of their frequency is not available.20.Saunier S. Salomon R. Antignac C. Nephronophthisis.Curr Opin Genet Dev. 2005; 15: 324-331Crossref PubMed Scopus (69) Google Scholar We could document five out of 56 NPHP1 patients (8.9%) presenting neurological signs that varied from JSRD and MTS to isolate severe tremor and with thickening of superior cerebellar peduncles. A more complex phenotype with hypotonia, developmental delay, ataxia, and central deafness was also found in one child. Overall, neurological defects in NPHP1 seem phenotypically wider than previously reported,16.Valente E.M. Marsh S.E. Castori M. et al.Distinguishing the four genetic causes of Jouberts syndrome-related disorders.Ann Neurol. 2005; 57: 513-519Crossref PubMed Scopus (81) Google Scholar and are, at the same time, more frequent than assumed. A major issue is the variability of clinical presentation. The two patients described here confirm the previously reported association between [NPHP1, DEL] and JSDR/MTS and extend to five times the number of cases reported in the literature. Interestingly, all of these cases (including the two cases reported here) presented a ‘milder’ form of JSDR, with moderate cerebellar vermis hypoplasia and elongation, but not thickening of superior cerebellar peduncles that seems to be a peculiar aspect of the association.13.Parisi M.A. Bennett C.L. Eckert M.L. et al.The NPHP1 gene deletion associated with juvenile nephronophthisis is present in a subset of individuals with Joubert syndrome.Am J Hum Genet. 2004; 75: 82-91Abstract Full Text Full Text PDF PubMed Scopus (186) Google Scholar,14.Castori M. Valente E.M. Donati M.A. et al.NPHP1 gene deletion is a rare cause of Joubert syndrome related disorders.J Med Genet. 2005; 42: e9Crossref PubMed Scopus (71) Google Scholar Therefore, a careful evaluation of brain MRIs, even in the absence of clear neurological involvement, such as in the case of simple arm tremor, is crucial for a correct clinical diagnosis. Moreover, molecular diagnostic testing in JSRD patients with renal involvement and NPHP1 deletion should always be investigated when such distinctive form of MTS is observed on brain MRI. The description of patients with intense tremor and thickened superior cerebellar peduncles in the absence of a clear MTS has never been reported so far and expands the clinical presentation of [NPHP1, DEL] patients. This phenotype is easily recognizable on clinical examination and could be part of the same clinical spectrum as JSRD. In fact, thickening of superior cerebellar peduncles could represent an even milder form of MTS, without obvious cerebellar vermis hypoplasia. However, this requires confirmation in additional cases. A single patient with [NPHP1, DEL] presented a complex syndrome consisting of hypotonia, ataxia, central hearing loss, and Leber congenital amaurosis associated with possible liver fibrosis. Leber congenital amaurosis and liver fibrosis are also part of the phenotypic spectrum of JSRD and central hearing loss has been occasionally reported as an associated feature, but brain imaging was surprisingly normal in the patient reported here. We cannot exclude that this patient could be affected by a yet undefined disorder that may be associated to [NPHP1, DEL] purely by chance. Overall, we have observed different neurological phenotypes associated with the same underlying molecular defect, that is, [NPHP1, DEL]. On the other hand, it is now evident that renal symptoms from alteration of urine concentrating potential to overt renal failure are frequent in patients with JSRD without [NPHP1, DEL] (manuscript in preparation), and therefore there is an overlap between JSRD with renal involvement (25%) and type 1 NPHP with MTS and/or tremor (9%). Recent papers confirm the association between NPHP and Joubert syndrome. Utsch et al.17.Utsch B. Sayer J.A. Attanasio M. et al.Identification of the first AHI1 gene mutations in nephronophthisis-associated Joubert syndrome.Pediatr Nephrol. 2006; 21: 32-35Crossref PubMed Scopus (66) Google Scholar identified the first NPHP and associated Joubert syndrome patient with AHI1 mutations. Two independent groups identified a new gene responsible for both nephrophthisis and Joubert syndrome; it encodes a large centrosomal protein named CEP290 that is involved in the control of ciliary activity.6.Valente E.M. Silhavy J.L. Brancati F. et al.Mutations in CEP290, which encodes a centrosomal protein, cause pleiotropic forms of Joubert syndrome.Nat Genet. 2006; 38: 623-625Crossref PubMed Scopus (283) Google Scholar,7.Sayer J.A. Otto E.A. O'Toole J.F. et al.The centrosomal protein nephrocystin-6 is mutated in Joubert syndrome and activates transcription factor ATF4.Nat Genet. 2006; 38: 674-681Crossref PubMed Scopus (395) Google Scholar Such ‘splitting and lumping’ raises a basic molecular question related to the pathogenesis of [NPHP1, DEL] overlapping syndromes. Although no definite answer is available at present, functional studies on the interaction between NPHP1-encoded nephrocystin and proteins involved in JSRDs such as Jouberin (encoded by the AHI1 gene) and CEP290 should be carried out to explain the clinical continuum between the above situations. Interestingly, nephrocystin and Jouberin share some structural homologies including an SH3 domain and several SH3 binding sites for protein–protein interaction, which are typical of proteins involved in signal transduction and cytoskeleton assembly. Louie and Glesson15.Louie C.M. Gleeson J.G. Genetic basis of Joubert syndrome and related disorders of cerebellar development.Hum Mol Genet. 2005; 14: R235-R242Crossref PubMed Scopus (67) Google Scholar recently proposed a model for cilia-based signalling in cerebellar development and potential role of Jouberin and nephrocystin. Although this possibility still requires experimental evidence, cilia have been recognized as crucial organelles present in distinct types of neurons as well as in renal tubule epithelial cells and are currently considered a basic mechanism for cyst transformation at this site (for a review see Menezes et al.21.Hildebrandt F. Otto E. Cilia and centrosomes: a unifying pathogenic concept for cystic kidney disease?.Nat Rev Genet. 2005; 6: 928-940Crossref PubMed Scopus (244) Google Scholar). The wide concept about an implication of ciliar proteins in cyst degeneration of the kidney is not confined to all identified NPHP genes, but may be extended to other renal cyst diseases such as dominant and recessive polycystic kidney disease,22.Menezes L.F. Cai Y. Nagasawa Y. et al.Polyductin, the PKHD1 gene product, comprises isoforms expressed in plasma membrane, primary cilium, and cytoplasm.Kidney Int. 2004; 66: 1345-1355Abstract Full Text Full Text PDF PubMed Scopus (107) Google Scholar Bardet–Biedl genes,23.Yen H.J. Tayeh M.K. Mullins R.F. et al.Bardet–Biedl syndrome genes are important in retrograde intracellular trafficking and Kupffer's vesicle cilia function.Hum Mol Genet. 2006; 15: 667-677Crossref PubMed Scopus (145) Google Scholar and Oro–Facial–Digital syndrome.24.Romio L. Fry A.M. Winyard P.J. et al.OFD1 is a centrosomal/basal body protein expressed during mesenchymal–epithelial transition in human nephrogenesis.J Am Soc Nephrol. 2004; 15: 2556-2568Crossref PubMed Scopus (116) Google Scholar Therefore, most of the renal diseases characterized by cysts are owing to mutations of genes coding for ciliary or centrosomal proteins that points to an unexpected convergence of mechanisms. In fact, many cysto proteins present coiled-coil domains and participate in protein-to-protein complexes. The basic mechanism of cilia implication in cyst transformation is not clear and few possibilities may be hypothesized.25.Watnick T. Germino G. From cilia to cyst.Nat Genet. 2003; 34: 355-356Crossref PubMed Scopus (131) Google Scholar The first is related to the mechanosensory function of cilia that is mainly supported by animal models of specific kidney gene inactivation.21.Hildebrandt F. Otto E. Cilia and centrosomes: a unifying pathogenic concept for cystic kidney disease?.Nat Rev Genet. 2005; 6: 928-940Crossref PubMed Scopus (244) Google Scholar Other implications of centrosomes and cystoproteins in cell cycle regulation and/or modulation of transcriptional pathways such as Wnt and AP1 may play additive functions.26.Germino G.G. Linking cilia to Wnts.Nat Genet. 2005; 37: 455-457Crossref PubMed Scopus (76) Google Scholar A second important question relates to the marked intrafamilial variability evident in our series. We excluded that such variability is owing to a different extension of the 2q13 deletion and alternative possibilities must be considered. An oligogenic model of disease transmission, in which multiple loci exert a synergistic effect to modify the expressivity of the disease trait, should be considered.27.Badano J.L. Katsanis N. Beyond Mendel: an evolving view of human genetic disease transmission.Nat Rev Genet. 2002; 3: 779-789Crossref PubMed Scopus (271) Google Scholar Bardet–Biedl syndrome, another autosomal-recessive disease, represents a very nice example of this.28.Badano J.L. Leitch C.C. Ansley S.J. et al.Dissection of epistasis in oligogenic Bardet–Biedl syndrome.Nature. 2006; 439: 326-330Crossref PubMed Scopus (206) Google Scholar Eleven genes have been identified,29.Chiang A.P. Beck J.S. Yen H.J. et al.Homozygosity mapping with SNP arrays identifies TRIM32, an E3 ubiquitin ligase, as a Bardet–Biedl syndrome gene (BBS11).Proc Natl Acad Sci USA. 2006; 103: 6287-6292Crossref PubMed Scopus (318) Google Scholar and in some patients, three mutations across two Bardet–Biedl syndrome loci interact to modify the onset and the severity of phenotype.30.Badano J.L. Kim J.C. Hoskins B.E. et al.Heterozygous mutations in BBS1, BBS2 and BBS6 have a potential epistatic effect on Bardet–Biedl patients with two mutations at a second BBS locus.Hum Mol Genet. 2003; 12: 1651-1659Crossref PubMed Scopus (151) Google Scholar This situation may explain the phenotypic variability observed in our patients. In conclusion, neurological defects varying from subtle cerebellar involvement to clear JSRD phenotypes are not so rare as expected in [NPHP1, DEL] patients and can be occasionally associated with retinal defects. The prevalence of such association justifies neurological and neuroradiological approaches in selected [NPHP1, DEL] patients. One hundred and twenty-five consecutive patients with intense polyuria and polydipsia were ascertained at the Nephrology Unit of G Gaslini Children Hospital over 10 years (1995–2004). For all patients, the diagnostic approach included clinical tests, ultrasound of kidneys and liver, and molecular tests to detect NPHP1 deletion and/or mutations. Fifty-six patients (44.8%) presented [NPHP1, DEL], whereas four patients were mutated (three with heterozygous NPHP1 deletion plus point NPHP1 mutation; one with NPHP1 compound heterozygous mutation).19.Caridi G. Dagnino M. Trivelli A. et al.Stop codon at arginine 586 is the prevalent nephronophthisis type 1 mutation in Italy.Nephrol Dial Transplant. 2006https://doi.org/10.1093/ndt/gfl277Google Scholar At the time of diagnosis, all these patients showed clinical and laboratory signs of NPHP, including urinary concentration defect (failure to increase fresh urine osmolality over 400 mOsm after desmopressin stimulation), increase of serum creatinine levels above the normal range for age, and small hyperechoic kidneys without corticomedullary differentiation. More than 50% patients had already developed ESRF and almost all with ESRF had been transplanted. Patients with possible neurological involvement underwent detailed neurological and ophthalmologic examination and instrumental testing, including brain MRI, EEG, graphology, evaluation of fundus oculi, and electroretinogram. Written informed consent for genetic studies was obtained from each patient or the legal tutor in the case of minors. NPHP1 homozygous deletion was tested by evaluating STS markers 804/6 and 9657T, mapping within NPHP1 intron 1 and MALL intron 1, respectively.8.Konrad M. Saunier S. Heidet L. et al.Large homozygous deletions of the 2q13 region are a major cause of juvenile nephronophthisis.Hum Mol Genet. 1996; 5: 367-371Crossref PubMed Scopus (122) Google Scholar, 9.Caridi G. Dagnino M. Miglietti N. et al.Juvenile nephronophthisis and related variants: clinical features and molecular approach.Contrib Nephrol. 2001; 136: 57-67Crossref PubMed Scopus (2) Google Scholar, 10.Hildebrandt F. Rensing C. Betz R. et al.Establishing an algorithm for molecular genetic diagnostics in 127 families with juvenile nephronophthisis.Kidney Int. 2001; 59: 434-445Abstract Full Text Full Text PDF PubMed Scopus (33) Google Scholar, 31.Heninger E. Otto E. Imm A. et al.Improved strategy for molecular genetic diagnostics in juvenile nephronophthisis.Am J Kidney Dis. 2001; 37: 1131-1139Abstract Full Text PDF PubMed Scopus (15) Google Scholar To evaluate the extension of the deletion 3′ of NPHP1 gene, new STS markers were generated within the LOC205251 gene by UCSC human genome database inspection and are listed in Table 2. Flanking regions between 9657T (located in MALL intron 1) and LOC205251 exon2 were not considered informative owing to the presence of segmental duplications and direct-inverted repeats. In all polymerase chain reaction experiments, an STS marker, external to the homozygous-deleted region, were co-amplified in order to test the presence of DNA sample. This work was supported by the Italian Ministry of Health (Ricerca Corrente, 2006). MD is supported by Fondazione Malattie Renali del Bambino. EMV acknowledge the Fondazione Pierfranco e Luisa Mariani ONLUS.
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