A stepwise pathway for biogenesis of 24-nt secondary siRNAs and spreading of DNA methylation
2008; Springer Nature; Volume: 28; Issue: 1 Linguagem: Inglês
10.1038/emboj.2008.260
ISSN1460-2075
AutoresLucia Daxinger, Tatsuo Kanno, Etienne Bucher, J. van der Winden, Ulf Naumann, A. J. M. Matzke, Marjori Matzke,
Tópico(s)Plant nutrient uptake and metabolism
ResumoArticle11 December 2008free access A stepwise pathway for biogenesis of 24-nt secondary siRNAs and spreading of DNA methylation Lucia Daxinger Lucia Daxinger Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Tatsuo Kanno Tatsuo Kanno Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Etienne Bucher Etienne Bucher Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, AustriaPresent address: Laboratory of Plant Genetics, University of Geneva, CH-1211 Geneva 4, Switzerland Search for more papers by this author Johannes van der Winden Johannes van der Winden Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Ulf Naumann Ulf Naumann Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Antonius J M Matzke Antonius J M Matzke Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Marjori Matzke Corresponding Author Marjori Matzke Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Lucia Daxinger Lucia Daxinger Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Tatsuo Kanno Tatsuo Kanno Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Etienne Bucher Etienne Bucher Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, AustriaPresent address: Laboratory of Plant Genetics, University of Geneva, CH-1211 Geneva 4, Switzerland Search for more papers by this author Johannes van der Winden Johannes van der Winden Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Ulf Naumann Ulf Naumann Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Antonius J M Matzke Antonius J M Matzke Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Marjori Matzke Corresponding Author Marjori Matzke Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria Search for more papers by this author Author Information Lucia Daxinger1,‡, Tatsuo Kanno1,‡, Etienne Bucher1, Johannes van der Winden1, Ulf Naumann1, Antonius J M Matzke1 and Marjori Matzke 1 1Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria ‡These authors contributed equally to this work *Corresponding author. Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Dr Bohr-Gasse 3, Vienna A-1030, Austria. Tel.: +43 1 79044 9810; Fax: +43 1 79044 9800; E-mail: [email protected] The EMBO Journal (2009)28:48-57https://doi.org/10.1038/emboj.2008.260 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We used a transgene system to study spreading of RNA-directed DNA methylation (RdDM) during transcriptional gene silencing in Arabidopsis thaliana. Forward and reverse genetics approaches using this system delineated a stepwise pathway for the biogenesis of secondary siRNAs and unidirectional spreading of methylation from an upstream enhancer element into downstream sequences. Trans-acting, hairpin-derived primary siRNAs induce primary RdDM, independently of an enhancer-associated 'nascent' RNA, at the target enhancer region. Primary RdDM is a key step in the pathway because it attracts the secondary siRNA-generating machinery, including RNA polymerase IV, RNA-dependent RNA polymerase2 and Dicer-like3 (DCL3). These factors act in a turnover pathway involving a nascent RNA, which normally accumulates stably in non-silenced plants, to produce cis-acting secondary siRNAs that induce methylation in the downstream region. The identification of DCL3 in a forward genetic screen for silencing-defective mutants demonstrated a strict requirement for 24-nt siRNAs to direct methylation. A similar stepwise process for spreading of DNA methylation may occur in mammalian genomes, which are extensively transcribed in upstream regulatory regions. Introduction Spreading of silent chromatin along a chromosome is a feature of many epigenetic processes in eukaryotes but the mechanisms, including the potential roles of non-coding RNAs, are still under investigation (Talbert and Henikoff, 2006; Clark, 2007; Pauler et al, 2007; Kwon and Workman, 2008). In fission yeast, an RNAi-mediated pathway fosters spreading of histone H3 lysine 9 methylation in heterochromatic DNA repeats (Locke and Martienssen, 2006; Iida et al, 2008; Zhang et al, 2008). The process of RNA-directed DNA methylation (RdDM) in Arabidopsis offers an opportunity to study spreading of cytosine methylation facilitated by the RNAi machinery and siRNAs in euchromatic portions of the genome. RNA-directed DNA methylation is a small RNA-mediated epigenetic modification that is highly developed in flowering plants (Bei et al, 2007). The hallmarks of RdDM include methylation of cytosines in all sequence contexts (CG, CNG, CNN, where N is A, T or C) and restriction of methylation to the region of RNA–DNA sequence homology. The establishment and maintenance of RdDM require for the most part conventional DNA cytosine methyltransferases, histone-modifying enzymes and nuclear-localized RNAi proteins (Matzke et al, 2007; Chan, 2008). In addition, several plant-specific proteins are required, most notably subunits of two novel RNA polymerases termed Pol IV and Pol V (Pikaard et al, 2008; Wierzbicki et al, 2008). Pol IV and Pol V share the same second largest subunit, NRPD2/NRPE2, but are distinguished by their unique largest subunits, NRPD1 and NRPE1, respectively. Pol IV is needed to produce and/or amplify the small RNA trigger, whereas Pol V acts downstream of this step to facilitate de novo methylation at the small RNA-targeted site (Pikaard et al, 2008). RdDM can silence transposons, but the potential reversibility of this process suggests broader functions in stress responses and plant development (Penterman et al, 2007; Chan, 2008; Hollick, 2008). To identify components of the RdDM machinery that are important for development, we established a two-component transgene silencing system based on an enhancer that is active in shoot and root meristem regions (Kanno et al, 2008). A forward genetic screen to recover mutants defective in RdDM of the target enhancer and silencing of a downstream GFP reporter gene has identified so far four dms (defective in meristem silencing) mutants. These include the previously identified SNF2-like factor DRD1 (DMS1) and the Pol V subunits, NRPE2a (DMS2) and NRPE1 (DMS5) (Kanno et al, 2004, 2005). Whether Pol V transcribes extensively in this transgene system or acts primarily to open chromatin at the siRNA-targeted site to expose DNA to cytosine methyltransferases is unknown. A recent study detected Pol V-dependent intergenic transcripts that may interact with siRNAs to induce methylation of homologous endogenous sequences (Wierzbicki et al, 2008). DMS3 is a novel structural-maintenance-of-chromosomes hinge domain-containing protein that has an unknown function in RdDM (Kanno et al, 2008). A distinctive feature of the meristem silencing system is the production of 24-nt secondary siRNAs that are associated with spreading of methylation downstream of the enhancer region originally targeted by primary siRNAs, a phenomenon termed transitivity (Voinnet, 2008). Secondary siRNA biogenesis is correlated with the disappearance of a longer 'nascent' transcript that overlaps with primary siRNAs. To explain these observations, we proposed a hypothetical pathway of secondary siRNA formation involving Pol IV as well as unidentified Argonaute (AGO), RNA-dependent RNA polymerase (RDR) and Dicer-like (DCL) activities (Kanno et al, 2008). Formation of approximately 21–22-nt secondary siRNAs and spreading of methylation within transcribed regions have been observed during post-transcriptional gene silencing (PTGS) in plants and shown to require RDR6 and transcription of the target gene (Vaistij et al, 2002; Van Houdt et al, 2003; Eamens et al, 2008). By contrast, our meristem silencing system provides a well-defined system for analysing the genetic requirements for the biogenesis of 24-nt secondary siRNAs and spreading of methylation (secondary RdDM) within upstream regulatory regions during transcriptional gene silencing (TGS). Here, we report the findings of experiments designed to investigate possible functions for two RNA-dependent RNA polymerases, RDR2 and RDR6, as well as NRPD1 and AGO4 in 24-nt secondary siRNA biogenesis, secondary RdDM and silencing of the GFP reporter gene. We also report the identification of a dcl3 mutant in a forward genetic screen. Results Secondary siRNA biogenesis, DNA methylation and 'nascent' RNA accumulation We used a reverse genetics approach to test several factors proposed to be involved in secondary siRNA biogenesis in our transgene TGS system (Figure 1A–C). RDR2 is needed to produce 24-nt 'heterochromatic' siRNAs (Xie et al, 2004); RDR6 is required to generate 21–22-nt secondary siRNAs in the PTGS pathway (Vaistij et al, 2002); AGO4 is needed for de novo DNA methylation (Chan et al, 2004) and for RNA slicing at some target loci (Qi et al, 2006); NRPD1, the largest subunit of Pol IV, is required for biogenesis of 24-nt heterochromatic siRNAs (Herr et al, 2005; Onodera et al, 2005). DCL3 is a dicer activity that produces 24-nt 'heterochromatic' siRNAs (Xie et al, 2004) and is discussed later in the context of a forward genetic screen. Figure 1.Transgene silencing system and hypothetical model for spreading of DNA methylation through secondary siRNAs. (A) The target locus (T) contains a meristem-specific enhancer (region targeted for methylation shown in black) placed upstream of a minimal promoter (hatched) and GFP-coding region. The unlinked silencer locus (S) contains a transcribed inverted DNA repeat of distal enhancer sequences encoding a hairpin RNA. The hairpin RNA trigger is diced into short interfering (si) RNAs, which induce de novo methylation of the target enhancer in trans. There is a short tandem repeat in the target enhancer (Kanno et al, 2008), which has an unknown function in spreading of methylation and silencing. 35Sp: 35S promoter of cauliflower mosaic virus. (B) Transgenic seedlings containing only the target locus (T) display GFP fluorescence in root and shoot apical meristem regions (RAM and SAM, respectively) (left); in seedlings containing the target and silencer loci (T+S), GFP fluorescence is abolished (right). (C) In the hypothetical model tested in this study, primary siRNAs (blue dashes) originating from DCL processing of the hairpin RNA trigger induce primary RdDM (blue 'm') of the targeted enhancer region (black box) and may guide AGO slicing of an overlapping nascent RNA (black arrow), which is proposed to be transcribed by Pol IV from the methylated DNA template. The Pol IV-generated nascent RNA, or cleavage fragments thereof, provide substrates for RDR; the resulting double-stranded RNA is diced to generate 24-nt secondary siRNAs (red dashes) that induce secondary RdDM (red 'm') in the downstream region (black shade). GFP expression is silenced. Primary and secondary RdDM require DRD1, Pol V and DMS3 (Kanno et al, 2008) and are presumably catalysed by the de novo methyltransferase DRM2 (Cao et al, 2003) with the participation of AGO4 (Chan et al, 2004). Download figure Download PowerPoint We introgressed the rdr2-1, rdr6-1, nrpd1-7 and ago4-1 mutations into the target (T)-silencer (S) line by crossing doubly homozygous plants (T/T;S/S) with the respective homozygous mutant (m/m). The resulting F1 progeny (genotype T/-; S/-; M/m) were self-fertilized to generate a segregating F2 population. To determine whether any of the mutants release GFP silencing, F2 seeds were sown on sterile medium and the number of GFP-positive seedlings was determined approximately 20 days after germination. If a mutation releases silencing, approximately one-third of the F2 progeny should be GFP positive; if a mutation does not release silencing, only around 18.5% of the F2 progeny should be GFP positive. With the exception of ago4-1, which appeared to partially release GFP silencing, none of the mutations alleviated silencing of the GFP reporter gene (Supplementary Table 1). We will first describe experiments with the three mutants that do not release GFP silencing: rdr2, rdr6 and nrpd1. To obtain rdr2, rdr6 and nrpd1 mutant plants and their wild-type siblings for the analysis of secondary siRNAs, DNA methylation and the nascent RNA, we genotyped F3 progeny to obtain the following genotypes: T/T; S/(S);m/m and T/T; S/(S);M/M. We used only F3 progeny that were homozygous for the target locus to avoid dosage effects on nascent RNA synthesis that might alter the amount of secondary siRNAs independently of any effect of a specific mutation. The silencer locus could be either homozygous or hemizygous, as in both states sufficient primary siRNAs are produced to induce GFP silencing. All wild-type and mutant plants harbouring the silencer locus contained primary siRNAs, which were present in three size classes (21-, 22- and 24-nt), that presumably result from multiple DCL enzymes acting on abundant hairpin RNAs in plants (Figure 2A) (Fusaro et al, 2006). By contrast, 24-nt secondary siRNAs were detected in wild-type plants and in the rdr6 mutant, but not in rdr2 and nrpd1 plants (Figure 2B). The genotypes of the rdr2, rdr6 and nrpd1 mutants were confirmed by testing for endogenous siRNA02, which is dependent on RDR2 and NRPD1 (Figure 2C), and tasiRNA255, which is dependent on RDR6 (Figure 2D). Figure 2.RNA profiles in rdr2, rdr6, nrpd1 and ago4 mutants. Northern blots of small RNAs: (A) primary siRNAs derived from hairpin RNA trigger; (B) secondary siRNAs; (C) siRNA02; (D) tasiRNA255. The arrowheads at the right indicate 21- and 24-nt-size classes. Ethidium bromide staining of the major RNA on the gels is shown as a loading control. The same blot was reprobed in B–D. (E) RT–PCR detection of nascent RNA; (F) RT–PCR of an actin control; (G) GFP expression. The DNA lane is amplified genomic DNA. Abbreviations: T/T;−/−, non-silenced plants containing a homozygous target locus; T/T;S/(S), silenced plants containing a homozygous target locus and either a hemizygous or homozygous silencer locus; WT, wild-type transgenic plant; Col-0, wild-type non-transgenic plants. For each of the four mutants tested (small letters), results from wild-type siblings (capital letters) are shown for comparison. The quantitative differences in primary siRNAs (A) are most likely due to the fact that RNA was prepared from pools of F2 or F3 plants that could be either homozygous or hemizygous for the silencer locus. Download figure Download PowerPoint The absence of the secondary siRNAs in the rdr2 and nrpd1 mutants was correlated with a strong reduction of secondary RdDM (Figure 3A and B). By contrast, secondary RdDM was present at wild-type levels in the rdr6 mutant (Figure 3C), which also contained a wild-type level of secondary siRNAs (Figure 2B). Primary RdDM remained at wild-type levels in the rdr2 and nrpd1 mutants (Figure 3A and B) and is apparently sufficient for silencing GFP expression, as indicated by the failure of rdr2 and nrpd1 mutations, which eliminate secondary siRNAs and secondary RdDM, to release GFP silencing (Supplementary Table 1). Figure 3.Analysis of DNA methylation at the targeted enhancer and downstream region by bisulphite sequencing in rdr2, rdr6, nrpd1 and ago4 mutants. The graphs show the percentage of methylation at individual cytosines in mutant plants (top) and their wild-type siblings (bottom). (A) rdr2/RDR2; (B) nrpd1/NRPD1; (C) rdr6/RDR6; (D) ago4/AGO4. The black bar represents the enhancer region targeted by primary siRNAs (primary RdDM); the shaded grey bar indicates the downstream region targeted by secondary siRNAs (secondary RdDM). Black lines: CG methylation; blue lines: CNG methylation; red lines: CNN methylation. Residual secondary RdDM in rdr2 may be due to low levels of 24-nt siRNAs produced by RDR6/DCL3 activities (Gasciolli et al, 2005; Eamens et al, 2008). The results are from at least 10 cloned sequences. Original data are shown in Supplementary Figure 4. Download figure Download PowerPoint The nascent RNA accumulates in the wild-type target line lacking the silencer locus but is greatly reduced or undetectable in rdr2, rdr6 and nrpd1 mutants and their wild-type siblings that contain silencer-encoded primary siRNAs (Figure 2E). In plants containing primary siRNAs and secondary siRNAs (wild-type plants and the rdr6 mutant), the absence of the nascent RNA can be explained by invoking the proposed turnover pathway involving AGO, RDR and DCL proteins (Figure 1C). This explanation does not account for the absence of the nascent RNA in nrpd1 and rdr2 mutants, which also lack secondary siRNAs despite wild-type levels of primary siRNAs and primary RdDM. One possibility is that the nascent RNA is cleaved by an AGO protein in the region overlapping with primary siRNAs. However, a 3′-cleavage fragment was not detected with the primers used (Figure 2E). An alternate explanation is that the nascent RNA is not transcribed in nrpd1 plants because of impaired Pol IVa function. Interestingly, the nascent RNA can be detected in the non-silenced target line in the nrpd1 background (Figure 2E), indicating that it is transcribed by an RNA polymerase other than Pol IV—presumably Pol II—in the absence of primary siRNAs and primary RdDM. A hypothetical explanation for the missing nascent RNA in the rdr2 mutant is that a Pol IV-generated transcript, or cleavage products thereof, is rapidly turned over by nuclear exonucleases, such as XRN2 and XRN3 (Gy et al, 2007), unless copied into double-stranded RNA by RDR2. The effects of the ago4-1 mutation on GFP silencing are complex because reactivation of GFP expression was not uniformly observed in both the root and shoot meristem regions of all seedlings. However, when considering seedlings in which reactivation occurred in both meristematic regions, there was a good correspondence between homozygosity for the ago4-1 mutation and a GFP-positive phenotype (Supplementary Table 1). A contribution of AGO4 to GFP silencing is consistent with the substantial loss of primary RdDM in ago4-1 plants (Figure 3D). The ago4-1 mutant lacked detectable secondary siRNAs (Figure 2B) and consequently had negligible secondary RdDM (Figure 3D); as expected from the absence of secondary siRNAs, accumulation of the nascent RNA was observed in ago4-1 plants (Figure 2E). Identification of a dcl3-null mutation in a forward genetic screen In a forward genetic screen using this transgene TGS system to identify silencing-defective mutants (Kanno et al, 2008), we identified one allele of a new complementation group, dms6. The dms6-1 mutant displayed an unusual pattern of accumulation of siRNA1003 (originating from 5S rDNA repeats) resembling that observed in a dcl3-1 mutant, which contains a T-DNA insertion in the DCL3 gene (Xie et al, 2004). The most striking feature of this pattern is an irregular ladder of RNAs that migrate above the 24-nt siRNA1003 observed in wild-type plants (Figure 4A). Given the similar patterns of siRNA1003 accumulation observed in dms6-1 and the known dcl3-1 mutant, we sequenced the DCL3 gene in the dms6-1 mutant and indeed detected a mutation that introduces a premature stop codon at amino acid 130, which is near the N-terminal part of the DCL3 protein (Figure 4, bottom); consequently, dms6-1, which is most likely a null allele of DCL3, has been renamed dcl3-5. Figure 4.RNA profile in the dcl3-5 mutant. Northern blots of small RNAs (A–C; E–J) and RT–PCR detection of the nascent RNA (D). (A) siRNA1003; (B) primary siRNAs; (C) secondary siRNAs; (D) nascent RNA (top) and actin control (bottom); (E) 45S rDNA siRNAs; (F) siRNA02; (G) solo LTR siRNAs; (H) Tag2 siRNAs; (I) Sat5 siRNAs. The arrowheads at the left indicate 21- and 24-nt size classes. In panels G–I, the lanes were pieced together from the same gel, as indicated by the dividing white lines. At the bottom of each blot, ethidium bromide staining of the major RNA on the gels is shown as a loading control. Bottom: domain structure of the DCL3 protein (1531 amino acids; Schauer et al, 2002) and position of the dcl3-5 mutation. It is unclear why a dcl3 mutation was recovered in this mutant screen and not a previous one based on silencing a seed-specific promoter (Kanno et al, 2004, 2005), but it may reflect the enhanced sensitivity of the present screen, which uses detection of enhanced GFP in colourless root tips. Download figure Download PowerPoint In the dcl3-5 mutant, 24-nt primary siRNAs derived from the hairpin RNA trigger are eliminated, whereas 21- and 22-nt primary siRNAs are present at slightly elevated levels (Figure 4B), probably through the compensatory action of DCL4 and DCL2, respectively (Fusaro et al, 2006). However, despite their increased abundance, the 21- and 22-nt siRNAs are unable to efficiently induce RdDM of the target enhancer region (Figure 5A). The dcl3-5 mutation also reduces methylation of endogenous sequences including a Tag2 transposon-related sequence (Figure 5B) and 5S rDNA repeats (Supplementary Figure 2). Figure 5.Analysis of DNA methylation at the targeted enhancer and an endogenous Tag2 element by bisulphite sequencing in the dcl3-5 mutant. The graphs show the percentage of methylation at individual cytosines in the dcl3-5 mutant (top) and wild-type plants (bottom) in the following sequences. (A) Targeted enhancer region (black bar) and downstream region (shaded grey bar); (B) Tag2 element. Black lines: CG methylation; blue lines: CNG methylation; red lines: CNN methylation. The results are from eight cloned sequences. Original data are shown in Supplementary Figure 4. The nearly complete loss of asymmetric CNN methylation in both sequences indicates a defect in de novo methylation; the residual CG and CNG methylation is probably due to siRNA-independent maintenance of symmetrical methylation (Aufsatz et al, 2002). Download figure Download PowerPoint Secondary siRNAs are undetectable in the dcl3-5 mutant (Figure 4C); however, it is unlikely that the absence of secondary siRNAs is a direct result of DCL3 deficiency, as initiation of secondary siRNA biogenesis depends on pre-existing primary RdDM, which is greatly reduced in the dcl3-5 mutant (Figure 5A). Nevertheless, the fact that the secondary siRNAs are 24-nt in length suggests that they are indeed products of DCL3 and able to induce methylation. The absence of secondary siRNAs is consistent with the presence of the nascent RNA in dcl3-5 plants (Figure 4D). We tested the accumulation of additional endogenous 24-nt siRNAs in the dcl3-5 mutant. A pattern of larger (>24-nt) siRNAs, similar to those observed for siRNA1003, as well as faint smaller siRNAs were observed from 45S rDNA repeats (Figure 4E). Other endogenous 24-nt siRNAs that are derived from sequences located in euchromatic chromosome arms, including siRNA02, a retrotransposon solo LTR and the Tag2 element analysed for methylation are mainly present as 21- and 22-nt size classes in dcl3-5 plants (Figure 4F–H). Interestingly, siRNAs derived from SAT5, a repetitive sequence family comprising several dispersed single copies in euchromatin and a tandem repeat block in the pericentromeric heterochromatin on chromosome 5 (L Daxinger and M Matzke, unpublished data) appear as prominent 21–22-nt siRNAs as well as a ladder of larger RNAs resembling those seen for siRNAs originating from 5S and 45S rDNA (Figure 4I). Discussion We have defined a stepwise pathway for biogenesis of 24-nt secondary siRNAs and unidirectional spreading of DNA methylation in Arabidopsis. Hairpin-derived primary siRNAs induce primary RdDM at the target enhancer region. This step initiates the Pol IV-RDR2-dependent turnover of a nascent RNA to produce secondary siRNAs, which trigger secondary RdDM in the downstream region (Figure 6). The stepwise scenario is supported by the observations that primary and secondary siRNA formation, as well as the corresponding RdDM steps, occur sequentially and can be uncoupled genetically. Figure 6.Stepwise pathway for secondary siRNA biogenesis and spreading of methylation. Proteins whose functions have been demonstrated in this study are shown in white letters on dark boxes. Top: in non-silenced plants containing the target locus (T) only, the targeted enhancer region (black box) is unmethylated and the nascent RNA, which normally does not interfere with GFP expression, is presumably transcribed by Pol II because it is still detectable in an nrpd1 mutant. Bottom: in the presence of the silencer locus (S), GFP expression is silenced by primary RdDM (blue 'm'), which is induced by hairpin-derived, DCL3-dependent 24-nt primary siRNAs (blue dashes). Primary RdDM attracts the secondary siRNA-generating machinery, which includes Pol IV, RDR2 and DCL3. In this model, Pol IV transcribes the methylated template to produce the nascent RNA, which is copied and diced by RDR2 and DCL3, respectively, to produce 24-nt secondary siRNAs (red dashes) that guide secondary RdDM (red 'm') in the downstream region (black shade). Pol IV may also transcribe the double-stranded RNA to sustain secondary siRNA production once the cycle is initiated (Supplementary Figure 2). In both cases, Pol IV transcribes a precursor of secondary siRNAs. Whether AGO cleavage of the nascent RNA is required to provide substrates for RDR2 is still not known. siRNA-guided de novo methylation requires DRD1, DMS3 and Pol V (Kanno et al, 2008) and is presumably catalysed by DRM2 (Cao et al, 2003). Download figure Download PowerPoint Our study supports a previous conjecture that primary RdDM is required to initiate 24-nt secondary siRNA formation. This requirement was initially suggested by the absence of secondary siRNAs in nrpe1 and drd1 mutants, which still produce primary siRNAs and the overlapping nascent RNA but lack primary RdDM (Kanno et al, 2008). The nascent RNA stably accumulates in the absence of primary RdDM in non-silenced plants but, in that instance, is presumably a Pol II transcript (Figure 6) because it is still made in an nrpd1 mutant. The key function of primary RdDM appears to be in attracting the secondary siRNA-generating machinery, which includes Pol IV and RDR2. The contribution may be direct if primary RdDM provides a template that is preferentially recognized by Pol IV, which has been proposed to transcribe methylated DNA (Herr et al, 2005; Onodera et al, 2005). In this model, Pol IV would transcribe the nascent RNA from the methylated template (Figure 6). An indirect contribution of primary RdDM is also possible if the nascent RNA needs to be sliced by AGO4 to provide substrates for RDR2. AGO4, which associates with WG/GW repeats in the C-terminal domain of NRPE1 (El-Shami et al, 2007), would be brought in together with Pol V during the primary RdDM step. Owing to the partial effect of the ago4-1 mutation on primary RdDM and the probable redundancy of AGO4 and AGO6 (Zheng et al, 2007), we could not discern whether AGO slicing of the nascent RNA is necessary for the biogenesis of secondary siRNAs. This issue can be tested in the future by using ago4 (and ago6) mutants deficient in catalytic PIWI domain but not the siRNA-binding PAZ domain (Qi et al, 2006). Whereas both of the RdDM steps require the same factors (Pol V-DRD1-DMS3-AGO4-DRM2) (Figure 6), the two DCL3-dependent 24-nt siRNA populations differ in their genetic requirements. Cis-acting secondary siRNAs, but not trans-acting hairpin-derived primary siRNAs, require Pol IV and RDR2 for their formation. Owing to the strong correlation between the appearance of secondary siRNAs and the disappearance of the nascent RNA, we infer that the nascent RNA is directly or indirectly transcribed by Pol IV and/or RDR2 during secondary siRNA formation. Secondary RdDM occurs only in the presence of secondary siRNAs, which are generated after primary RdDM is induced. Primary RdDM can be established and maintained independently of secondary RdDM, as observed in rdr2 and nrpd1 mutants. The dispensability of RDR2 (and RDR6) for primary RdDM differs from the situation in fission yeast, where heterochromatin formation induced in trans by hairpin-derived siRNAs nevertheless requires RDR-dependent synthesis of double-stranded RNA in cis at the target locus (Iida et al, 2008). The basis of this difference is unknown but, as discussed below, it may reflect whether siRNAs interact with target DNA or RNA sequences. Although the nascent RNA is clearly involved in the formation of secondary siRNAs that induce secondary RdDM, our data suggest that it does not contribute to primary RdDM or GFP silencing. The evidence for this is that the nascent RNA is greatly reduced
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