RECENT ADVANCES IN RATIONAL APPROACHES FOR ENZYME ENGINEERING
2012; Elsevier BV; Volume: 2; Issue: 3 Linguagem: Inglês
10.5936/csbj.201209010
ISSN2001-0370
AutoresKerstin Steiner, Helmut Schwab,
Tópico(s)Enzyme Structure and Function
ResumoEnzymes are an attractive alternative in the asymmetric syntheses of chiral building blocks. To meet the requirements of industrial biotechnology and to introduce new functionalities, the enzymes need to be optimized by protein engineering. This article specifically reviews rational approaches for enzyme engineering and de novo enzyme design involving structure-based approaches developed in recent years for improvement of the enzymes’ performance, broadened substrate range, and creation of novel functionalities to obtain products with high added value for industrial applications.
Referência(s)