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A Fast and Easy Method for Chromosome Karyotyping in Adult Teleosts

1974; Volume: 36; Issue: 3 Linguagem: Inglês

10.1577/1548-8659(1974)36[169

1548-8640

John R. Gold,

Reproductive biology and impacts on aquatic species

Although both methods are employed with varying success, each suffers from disad­vantages when large numbers of well-spread metaphases are desired from a large sample of adult individuals. For example, tissue squashes· often result in low numbers of poorly spread mitotic preparations and in addition require some degree of manipulatory dexterity. Cell cul­ture techniques, on the other hand, while pro­viding excellent spread numbers and resolution of chromosome detail, require laboratory skills and equipment not always available. Moreover, the cost of cell culture procedures necessary for karyotyping a large sample of adult fish would preclude its use in all but very specialized lab­oratories. At the Davis trout genetics laboratory, the possibility of using karyotypic differences as racial and/or species markers among salmonid fish is being pursued. Since a large number of adult individuals are examined, ~rnate meth­ods of chromosome karyotyping have been sought which are both inexpensive and simple in design and execution. The procedure de­scribed below has satisfied both criteria, and is currently employed as the sole method of chro­mosome karyotyping at the Davis laboratory. MATERIALS AND METIIODS