Portal de Periódicos da CAPES

Heteroduplex rejection during single-strand annealing requires Sgs1 helicase and mismatch repair proteins Msh2 and Msh6 but not Pms1

2004; National Academy of Sciences; Volume: 101; Issue: 25 Linguagem: Inglês

10.1073/pnas.0305749101

1091-6490

Neal Sugawara, Tamara Goldfarb, Barbara Studamire, Eric Alani, James E. Haber,

RNA and protein synthesis mechanisms

Recombination between moderately divergent DNA sequences is impaired compared with identical sequences. In yeast, an HO endonuclease-induced double-strand break can be repaired by single-strand annealing (SSA) between flanking homologous sequences. A 3% sequence divergence between 205-bp sequences flanking the double-strand break caused a 6-fold reduction in repair compared with identical sequences. This reduction in heteroduplex rejection was suppressed in a mismatch repair-defective msh6 Δ strain and partially suppressed in an msh2 separation-of-function mutant. In mlh1 Δ strains, heteroduplex rejection was greater than in msh6 Δ strains but less than in wild type. Deleting PMS1, MLH2 ,or MLH3 had no effect on heteroduplex rejection, but a pms1 Δ mlh2 Δ mlh3 Δ triple mutant resembled mlh1 Δ. However, correction of the mismatches within heteroduplex SSA intermediates required PMS1 and MLH1 to the same extent as MSH2 and MSH6 . An SSA competition assay in which either diverged or identical repeats can be used for repair showed that heteroduplex DNA is likely to be unwound rather than degraded. This conclusion is supported by the finding that deleting the SGS1 helicase also suppressed heteroduplex rejection.