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Effects of cytochalasin B on polymorphonuclear leucocyte locomotion, phagocytosis and glycolysis

1972; Elsevier BV; Volume: 73; Issue: 2 Linguagem: Inglês

10.1016/0014-4827(72)90062-6

1090-2422

Sally H. Zigmond, James G. Hirsch,

Lipid Membrane Structure and Behavior

Observations have been made on the inhibitory action of cytochalasin B (CB) on locomotion, phagocytosis and glycolysis in human, horse, and rabbit polymorphonuclear leucocytes (PMNs). Locomotion in all three species was suppressed by as little as 0.3 μg/ml CB and completely blocked by 3 μg/ml, as judged by direct observation or by a Millipore filter assay system. Phagocytosis was evaluated morphologically as well as in a bactericidal system. Three μg/ml CB suppressed, but did not completely block, phagocytosis by horse and human cells; increasing the concentration of CB to 40 μg/ml exerted little additional suppression of phagocytic capacity. Rabbit exudate cells ingested and killed certain species of bacteria Staph. aureus or Salmonella less effectively than did horse or human cells; in these instances CB did not alter the killing rate significantly CB did inhibit the killing of E. coli by rabbit cells. Three μg/ml CB resulted in a partial inhibition of several glycolytic parameters, i.e., glucose utilization, lactate production, and CO2 production from C-1 or C-6 labeled glucose. These parameters were inhibited more than 75 % by 10 μg/ml CB. Studies made thus far indicate that CB probably suppresses glycolysis by interfering with glucose transport or phosphorylation. Comparison of the inhibition of various parameters by CB and by other metabolic inhibitors suggests that the glycolytic inhibition by CB is probably not the primary drug action responsible for the altered locomotion and phagocytosis.