Changes in Organization of the Endoplasmic Reticulum during Xenopus Oocyte Maturation and Activation
2001; American Society for Cell Biology; Volume: 12; Issue: 4 Linguagem: Inglês
10.1091/mbc.12.4.1103
ISSN1939-4586
AutoresMark Terasaki, Linda L. Runft, Arthur R. Hand,
Tópico(s)Pancreatic function and diabetes
ResumoThe organization of the endoplasmic reticulum (ER) in the cortex ofXenopus oocytes was investigated during maturation and activation using a green fluorescent protein chimera, immunofluorescence, and electron microscopy. Dense clusters of ER developed on the vegetal side (the side opposite the meiotic spindle) during maturation. Small clusters appeared transiently at the time of nuclear envelope breakdown, disappeared at the time of first polar body formation, and then reappeared as larger clusters in mature eggs. The appearance of the large ER clusters was correlated with an increase in releaseability of Ca 2+ by IP 3 . The clusters dispersed during the Ca 2+ wave at activation. Possible relationships of ER structure and Ca 2+ regulation are discussed.
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