Portal de Periódicos da CAPES

The function of SECIS RNA in translational control of gene expression in Escherichia coli

2002; Springer Nature; Volume: 21; Issue: 24 Linguagem: Inglês

10.1093/emboj/cdf673

1460-2075

Martin Thanbichler,

RNA modifications and cancer

Article16 December 2002free access The function of SECIS RNA in translational control of gene expression in Escherichia coli Martin Thanbichler Martin Thanbichler Department of Biology I, Microbiology, University of Munich, Maria-Ward-Straße 1a, D-80638 Munich, Germany Search for more papers by this author August Böck Corresponding Author August Böck Department of Biology I, Microbiology, University of Munich, Maria-Ward-Straße 1a, D-80638 Munich, Germany Search for more papers by this author Martin Thanbichler Martin Thanbichler Department of Biology I, Microbiology, University of Munich, Maria-Ward-Straße 1a, D-80638 Munich, Germany Search for more papers by this author August Böck Corresponding Author August Böck Department of Biology I, Microbiology, University of Munich, Maria-Ward-Straße 1a, D-80638 Munich, Germany Search for more papers by this author Author Information Martin Thanbichler1 and August Böck 1 1Department of Biology I, Microbiology, University of Munich, Maria-Ward-Straße 1a, D-80638 Munich, Germany *Corresponding author. E-mail: [email protected] The EMBO Journal (2002)21:6925-6934https://doi.org/10.1093/emboj/cdf673 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The incorporation of selenocysteine into proteins is directed by specific UGA codons and mRNA secondary structures, designated SECIS elements. In bacteria, these elements are positioned within the reading frame of selenoprotein mRNAs immediately downstream of the triplet coding for selenocysteine, and they tether a complex of the selenocysteine-specific elongation factor SelB, GTP and selenocysteyl-tRNASec to the site of UGA decoding. A SECIS-like structure was identified in the 5′ non-translated region of the selAB transcript, encoding selenocysteine synthase and SelB. It specifically binds to SelB and the formation of a SelB·GTP·selenocysteyl-tRNASec complex on the SECIS-like element represses expression of the downstream gene. This effect is abolished by mutations preventing formation of the complex. The regulatory pattern observed correlated with the levels of sel gene products. As quaternary complex formation on the SECIS-like element did not influence the transcription rate and only slightly reduced the level of selAB mRNA, it was concluded that the structure is involved in regulating translation initiation efficiency, thereby coupling selenocysteine biosynthesis to the availability of the trace element selenium. Introduction The amino acid selenocysteine (Sec) has been identified as a component of selected proteins in bacteria, archaea and eukarya (Low and Berry, 1996; Stadtman, 1996; Rother et al., 2001). It is encoded by an in-frame UGA codon interrupting the reading frame of the respective selenoprotein gene and is co-translationally inserted into the nascent polypeptide chain. In Escherichia coli, there are three genes coding for selenium-containing formate dehydrogenases, which are expressed constitutively (fdoG) or under anaerobic conditions only (fdhF and fdnG) (Zinoni et al., 1986; Berg et al., 1991; Sawers et al., 1991). The biosynthesis of selenocysteine and its insertion into these proteins require the function of at least four gene products (see Figure 10). selC codes for tRNASec, which is charged with L-serine by seryl-tRNA synthetase (SerS) and serves as the adapter at which the seryl moiety is converted into the selenocysteyl derivative. The conversion reaction is catalyzed by selenocysteine synthase (the selA gene product) with the aid of monoselenophosphate as the selenium donor substrate, which is synthesized from selenide and ATP by selenophosphate synthetase (the selD gene product). SelB, finally, is a specialized translation factor taking over the function of elongation factor (EF) Tu in selenocysteine insertion. Its N-terminal three domains are homologous to EF-Tu, but it additionally exhibits a C-terminal extension of 272 amino acids, designated domain IV (for reviews, see Hüttenhofer and Böck, 1998; Böck, 2001; Böck et al., 2002). SelB, in addition to interacting with guanosine nucleotides, binds two RNA ligands, namely Sec-tRNASec and the SECIS element of the mRNAs coding for selenoproteins. In bacteria, this mRNA structure is located immediately 3′ of the UGA codon that determines the position of selenocysteine incorporation in the nascent polypeptide chain. The binding order is random, but interaction with both RNA substrates leads to a stabilization of the resulting quaternary complex consisting of SelB, GTP, Sec-tRNASec and the SECIS element (Baron et al., 1993). Using rapid kinetic analysis, it was shown that the affinity of SelB for the SECIS element is maximized by Sec-tRNASec and decreases after its dissociation to allow the translation of codons 3′ of the UGA directing selenocysteine insertion (Thanbichler et al., 2000). The interaction of SelB with the bacterial SECIS element has been additionally probed with a variety of techniques, like truncation of fdhF from the 3′ side (Zinoni et al., 1990), introduction of single or multiple mutations (Heider et al., 1992; Liu et al., 1998), gel retardation experiments (Baron et al., 1993; Kromayer et al., 1996), footprint analysis (Ringquist et al., 1994) and in vitro selection of RNA molecules (aptamers) that tightly bind to SelB (Klug et al., 1997,1999). The main information gained and relevant for this communication was that: (i) the interaction involves a 17 kDa C-terminal domain of SelB (SelB472–614), designated domain IVb, and the apical part of the SECIS hairpin; (ii) the affinity of the interaction was lower between SelB and the SECIS element as compared with that between domain IVb and the SECIS element, but was equal when SelB was additionally complexed with Sec-tRNASec; (iii) finally, and intriguingly, some of the aptamers selected in vitro displayed excellent binding affinities but were not functional in the decoding process. Therefore, binding and function in the decoding process could be separated. In the present communication, we report the existence of a SECIS element present in the 5′ untranslated region of the mRNA for the bicistronic selAB operon. We show that this SECIS structure interacts with SelB and, in the presence of Sec-tRNASec, represses translation. By this novel mechanism, it adjusts the level of the gene products to the supply of the trace element selenium. Interestingly, this regulatory SECIS element is functional in the decoding process, although at reduced efficiency. Results The SECIS-like element The structural genes coding for selenocysteine synthase (selA) and translation factor SelB (selB) in E.coli are organized in a bicistronic transcriptional unit and their reading frames overlap by four bases (Figure 1A). Previous analysis of the transcription of the selAB operon has shown that it is transcribed very weakly from a single start site at position −48 relative to the selA initiation codon (Sawers et al., 1991). When the 5′ non-translated RNA sequence was inspected for regulatory motifs, a putative RNA secondary structure was detected that strikingly resembled the SECIS element directing selenocysteine insertion (Figure 1B). Using mfold 3.1 (Mathews et al., 1999), its free energy was calculated to be −10.6 kcal/mol at 37°C. Since it is located outside the reading frame, it was designated SECIS-like. The putative minimal binding region of the SECIS-like element contains the essential structural and sequence elements that had been shown to be required for SECIS proper (Heider et al., 1992; Hüttenhofer et al., 1996; Liu et al., 1998,Li et al., 2000): the apical helix is closed by two G–C/C–G Watson–Crick base pairs and a preceding A–U/U–A base pair at the basal end and a C–G base pair at the distal end, and contains a bulged-out U residue at a position equivalent to +17 in the SECIS elements. The loop region contains the GU doublet that is supposed to make contact with the SECIS-binding pocket of SelB (Figure 1C). Figure 1.(A) Organization of the selAB operon. (B) Fold of the stem–loop structure at the 5′ end of the selAB transcript as calculated by the mfold 3.1 program (Mathews et al., 1999). The transcription initiation site as determined by Sawers et al. (1991), the Shine–Dalgarno motif (SD), the selA start codon (*) and the exchanges introduced to generate a mutant form of the SECIS-like element are indicated. (C) Comparison of the SECIS-like element with the SECIS structures of the fdhF and fdnG mRNAs. The minimal binding regions are depicted in bold. Bases supposed to make direct contact with SelB (Hüttenhofer et al., 1996) are shaded. The bulged-out U of the SECIS elements at position +17 relative to the selenocysteine-encoding UGA codon is indicated. Download figure Download PowerPoint The SECIS-like structure is positioned at the immediate 5′ end of the mRNA and does not include the Shine–Dalgarno motif (Figure 1B). The SECIS-like element binds SelB and forms a complex with SelB and Sec-tRNASec The striking similarities between the SECIS and the SECIS-like element prompted the analysis on whether the SECIS-like element is a binding target for translation factor SelB. A transcript was synthesized in vitro comprising the stem–loop structure at the 5′ end of the selAB mRNA (see Figure 1B). It was 32P-labeled at the 5′-terminus and used in gel retardation experiments (Figure 2). Figure 2A shows that the SECIS-like element yields a distinct shift signal at a 5-fold or higher molar excess of protein over the transcript despite the presence of a huge excess of unspecific competitor tRNA. The signal remains unchanged when additional competitor tRNA is added, but disappears completely if an equal amount of cognate SECIS RNA is included in the reactions. This indicates a specific interaction that involves the SECIS-binding site of SelB. Figure 2.Interaction of the SECIS-like element with wild-type SelB (A) and SelB472–614 (B). 32P-labeled SECIS-like element (80 nM) was incubated with increasing amounts of protein in the presence of 3.6 μM yeast tRNA. In the last two lanes, additional 3.6 μM unspecific (yeast tRNA) or specific (fdhF SECIS) competitor RNA was added to the reactions. After separation of the mixtures in a non-denaturing polyacrylamide gel, radioactivity was detected using a PhosphorImager. Download figure Download PowerPoint From previous experiments, it was known that a crucial requirement for SelB to function in the decoding of UGA is the capacity to undergo a sequence of conformational changes which ultimately result in the release of Sec-tRNASec at the ribosomal A-site and in the resolution of the SelB·SECIS complex (Thanbichler et al., 2000). These switches are reflected in the fact that SelB interacts with SECIS elements with a lower affinity and specificity, as does the C-terminal binding domain alone. To assess whether the SECIS-like element follows the same pattern, mobility shift experiments were conducted by probing the SECIS-like transcript with SelB472–614 (Figure 2B). Surprisingly, SelB472–614 is unable to form a stable complex with the SECIS-like element, whereas it tightly binds the fdnG (data not shown) and fdhF SECIS elements (Kromayer et al., 1996; Thanbichler et al., 2000). A weak interaction certainly takes place, as indicated by the tailing of the signal for the transcript, which is absent in the lane containing an excess of specific competitor RNA. There was no interaction detectable between the SECIS-like element and isolated SelB379–614, which comprises almost the entire domain IV of SelB (data not shown). These results are different from those obtained with the fdnG SECIS element, which binds to SelB379–614 with the same affinity as to full-length SelB (our unpublished results). The characteristics of the SECIS-like element are reminiscent of the binding behavior of some of the aptamers selected in vitro by Klug et al. (1997), which interact well with full-length SelB, but not with isolated SelB472–614. When Sec-tRNASec is included in the incubation mixture (Figure 3B), a strong retardation signal is already present at a 2.5-fold molar excess of protein, indicating a significant increase in the affinity of SelB for the SECIS-like element. Moreover, the mobility of the complex formed is enhanced, which is due to the additional negative charges introduced by binding of the tRNA. This resembles the binding behavior described previously for the fdhF SECIS element; namely, that a tighter and fast migrating complex is formed when both nucleic acid substrates are bound (Baron et al., 1993). Figure 3.Effect of Sec-tRNASec on complex formation between SelB and the SECIS-like element. 32P-labeled SECIS-like element (80 nM) was incubated with increasing concentrations of SelB in the presence of 3.6 μM yeast tRNA (A). To analyze the influence of Sec-tRNASec, 1.0 μM Sec-tRNASec and 100 μM GTP were additionally included in the reactions (B). GTP (100 μM) was added to the gel and electrophoresis buffer for separating the mixtures in both cases. Radioactivity was detected using a PhosphorImager. The positions of the ternary (a) and quaternary (b) complexes are indicated by arrows. Download figure Download PowerPoint To test whether the interaction between SelB and the SECIS-like element is specific, we have introduced two base changes into the loop region of the hairpin (see Figure 1B). From previous experiments, it was known that the exchange of bases in the terminal loop of the fdhF SECIS element is detrimental to SelB binding and thus abolishes its function in the decoding process (Heider et al., 1992; M.Kromayer, unpublished results). Figure 4 indicates that this also holds for the binding capacity of the mutant SECIS-like element, as the ability to interact with SelB disappears. Figure 4.Specificity of the interaction between the SECIS-like element and SelB. 32P-labeled wild-type (WT) or mutant (AA) SECIS-like element (80 nM) (see Figure 1B) was mixed with increasing amounts of SelB in the presence of 3.6 μM yeast tRNA as unspecific competitor. Complexes formed were separated from unbound RNA by electrophoresis in a non-denaturing polyacrylamide gel and radioactivity was detected using a PhosphorImager. Download figure Download PowerPoint To obtain qualitative information on the affinity of the interaction between SelB and the SECIS-like element, in vitro transcripts of the SECIS-like element and the fdnG SECIS element were titrated in parallel with increasing amounts of SelB. Complex formation, as analyzed by gel retardation, is depicted in Figure 5. It appears that the SECIS-like structure has an ∼3- to 4-fold lower affinity to the target protein. The KD value for the interaction of the fdnG SECIS with SelB has been determined to be 1.4 nM (Thanbichler et al., 2000). Therefore, the corresponding value for the binding of the SECIS-like element might be in the range of ∼10 nM. Figure 5.Affinity of the SECIS-like element for SelB. 32P-labeled fdnG SECIS or SECIS-like element (80 nM) was incubated with increasing amounts of SelB in the presence of 3.6 μM yeast tRNA as unspecific competitor. After separation in a non-denaturing polyacrylamide gel, unbound and complexed RNA was detected using a PhosphorImager. Download figure Download PowerPoint The SECIS-like element functions in the regulation of selAB expression The characteristics of the SECIS-like element described above showed convincingly that it can form both a complex with SelB alone and a quaternary complex with SelB, Sec-tRNASec and presumably GTP, the latter interaction being significantly more stable. A speculation emanating from this is that the formation of the quaternary complex on the SECIS-like structure might affect the expression of the selAB mRNA. To follow this assumption, we have correlated the capacity of cells to form this complex with the amount of gene products for the sel genes. To this end, the wild-type strains PT90422, MC4100 and EMG29, and derivatives thereof carrying mutations in one or two of these genes, were grown to exponential phase, the cells were harvested and lysed, and the lysates analyzed for the quantity of sel gene products by immunoblotting (Figure 6). Intriguingly, a full correlation was detected: synthesis of the selA gene product was suppressed in a genetic background that renders the cells proficient for the formation of Sec-tRNASec and the concomitant production of SelB. In contrast, mutations in either selD, selC, selB or selA alleviate this suppression. Figure 6.Immunoblot analysis of the intracellular level of SelA, SelB and SelD in sel mutants derived from E.coli strains PT90422, MC4100 and EMG29. Cells of the strains PT90422 (wild type), MTA3 (ΔselB), MTA1 (ΔselA), MTA2 (ΔselC), MTA12 (ΔselA, ΔselC), AF90422 (ΔselAB), MC4100 (wild type), WL400 (ΔselD), FM460 (ΔselC), EMG29 (wild type), MB08 (selD165) and GY01 (selA160) were grown to an OD600 of 1.5, harvested and lysed in sample buffer. The lysates were applied to a 10% SDS–polyacrylamide gel. After separation of the proteins and transfer to a nylon membrane, immunoblot analysis was performed. Note that the point mutation carried by strain GY01 results in the synthesis of a stable but inactive form of SelA, whereas all other mutants do not synthesize stable products of the mutated genes. Download figure Download PowerPoint The same regulatory pattern was observed for the selB gene product, although the effect was less pronounced. This correlates with previous results demonstrating that there is an internal translation initiation site upstream of the selB gene, which at least partly uncouples selB from selA mRNA translation and enables expression of selB when transcribed as a single gene under the control of the T7Φ10 promoter (Forchhammer et al., 1990). Physiologically, this is meaningful since SelB represents the key regulatory component, whose concentration should not fall below a critical level. The level of SelD remained constant in all strains tested and seems not to be regulated. Analysis of regulation by the SECIS-like element employing lacZ reporter gene fusions Since immunoblotting yields information on the steady-state level of gene products only and does not integrate the contribution of synthesis and degradation, the studies on the regulatory function of the SECIS-like element were extended by the use of lacZ reporter gene fusions. Both transcriptional and translational fusions were constructed (Figure 7A). To ensure the correct stoichiometry between regulatory factors and the mRNA, the fusion constructs were integrated into the λ phage and subsequently transferred in single copy to the λ attachment site of the chromosome. Assaying the level of β-galactosidase in cells lysogenized with λRL1, which carries the selA upstream region and a 5′ fragment of selA fused in-frame to lacZ, shows that the expression of the reporter gene is repressed in a wild-type genetic background, whereas it is increased ∼5-fold in strains with a mutation preventing formation of a functional quaternary complex (Figure 7B). The results obtained fit the regulation pattern as revealed by immunoblot analysis (see Figure 6) and indicate that binding of SelB·GTP·Sec-tRNASec to the SECIS-like element is crucial for the repression observed in the wild-type strain. This model is corroborated by the fact that only the derepressed level of β-galactosidase is measured in strains carrying λRL12, a phage whose insert is identical to that of λRL1 except for two mutations in the loop region of the SECIS-like element that have been shown to prevent binding of SelB (see Figure 4). The regulatory pattern of selB expression was followed using strains that were lysogenized with phage λRLΔAB carrying the selA upstream region, the selA open reading frame and a 5′ fragment of selB fused in-frame to lacZ. A deletion that has been shown not to have any polar effects on selB expression (data not shown) was introduced into the selA gene of the fusion construct to prevent complementation in a ΔselA genetic background. As depicted in Figure 7C, synthesis of β-galactosidase is repressed ∼3-fold in the wild type, whereas derepression occurs in strains deficient in quaternary complex formation. The effect is less distinct than the one measured for selA and again corresponds to the observations made by immunoblot analysis (Figure 6). Figure 7.Regulatory role of the SECIS-like element. (A) Reporter constructs employed to study regulation by the SECIS-like element. The restriction sites used to insert the respective fragment into plasmids pRS551 or pRS552 (Simons et al., 1987) are shown. The position of the deletion introduced into selA on the reporter construct harbored by phage λRLΔAB is indicated (Δ). λRL12 and λRC12 carry the mutated SECIS-like element (see Figure 1B), whereas all other phages contain the wild-type sequence. (B) β-galactosidase activities measured for strains PT90422 (WT), MTA1 (ΔselA), MTA2 (ΔselC) and MTA3 (ΔselB) after lysogenization with phages that carry selA′-′lacZ translational fusions encoding the wild-type (λRL1) or mutant (λRL12) SECIS-like element. (C) β-galactosidase activities determined for the same strains, when carrying the selAB′-′lacZ translational fusion harbored by λRLΔAB. (D) β-galactosidase activities exhibited by strains PT90422, MTA1, MTA2 and MTA3, when lysogenized with phages λRC1 and λRC12, which harbor transcriptional fusions encoding the wild-type and mutant SECIS-like element, respectively. Download figure Download PowerPoint To ensure that the increase in β-galactosidase production that was measured in the mutant strains is a specific effect caused by the absence of a factor necessary to form the regulatory complex, complementation studies were performed. It was shown that the introduction of plasmids that lead to a moderate overproduction of SelA (with the SECIS-like element deleted from the upstream region of the plasmid-encoded selA gene) or tRNASec could completely override the derepression of reporter gene expression that was observed with strains MTA1::λRL1 (ΔselA) or MTA2::λRL1 (ΔselC), respectively, when they were carrying a control plasmid only (data not shown). Effect on selAB mRNA synthesis and stability There are several possible mechanisms that could underly the observed regulatory influence exerted by quaternary complex formation at the SECIS-like element, namely transcriptional attenuation, modulation of mRNA stability or regulation of translation initiation. Regulation of transcription by binding of proteins to their own message has emerged to be a general scheme for adjusting protein biosynthesis to the level of a gene product (Yanofsky, 2000). To analyze whether the SECIS-like element might mediate attenuation of selAB transcription, the level of β-galactosidase was assayed in strains lysogenized with phage λRC1, which carries the selA upstream region fused to the intact lacZ gene (Figure 7D). It appeared that the level of the reporter gene product was constant, independent of the genotype of the infected cells. The same specific β-galactosidase activity was obtained when the wild-type strain PT90422 was lysogenized with λRC12 instead of λRC1. This phage carries a similar construct with a mutated form of the SECIS-like element unable to interact with SelB (see Figure 4). These results clearly exclude a regulatory role for the SECIS-like element at the transcriptional level. Next, it was investigated whether the stability of the selAB mRNA changes when the SECIS-like element is included in a quaternary complex. Many mRNAs have been shown to be protected against degradation by secondary structures positioned at their immediate 5′ ends, which are supposed to interfere with the initial endonucleolytic cleavage catalyzed by RNase E (Rauhut and Klug, 1999). Accordingly, binding of SelB·GTP· Sec-tRNASec might alter the conformation of the the SECIS-like element or the bordering region and thereby enhance the degradation of the selAB mRNA. To address this issue, the steady-state level and half-life of the selAB mRNA were analyzed using the S1 nuclease protection assay. Two labeled probes (Figure 8A) hybridizing with the 5′ and the 3′ end of the selA transcript, respectively, were synthesized and used to quantify the transcript levels. As depicted in Figure 8B, there was a reproducible, ∼1.5- to 2-fold increase in the steady-state amount of mRNA in the ΔselC mutant as compared with the wild-type strain. A similar effect was also observed in a ΔselA or ΔselB genetic background (data not shown). The ratios between the levels of the 3′ and the 5′ ends remained approximately constant at 60%. To investigate whether the half-life of the transcript changes under conditions of impeded quaternary complex formation, the decay profile of the selAB mRNA was determined for PT90422 (wild type) and MTA2 (ΔselC), which both have the wild-type operon. Figure 8C shows that the 5′ end of the transcript is rapidly degraded and reaches a basal level ∼6 min after inhibition of transcription. In the ΔselC genetic background, the mRNA is more stable (t1/2 = 67 s) than in the wild-type strain (t1/2 = 52 s), which explains the difference in the steady-state levels observed before. Very similar values were obtained with probe A2, which anneals to the 3′ region of selA (data not shown). Figure 8.Steady-state level and half-life of the selAB transcript. (A) Localization of probes A1 and A2 directed against the selA mRNA. The restriction sites used to generate the probes are indicated. (B) S1 nuclease protection assay. Seventy-five micrograms of yeast tRNA or total RNA that was extracted from cells of strains PT90422 (WT) and MTA2 (ΔselC) harvested before (0 min) or 2, 4 and 6 min after inhibition of transcription by addition of rifampicin were incubated with 10 000 c.p.m. of each probe. After treatment with S1 nuclease, the remaining probes were applied to an 8% urea–polyacrylamide gel, separated and visualized using a PhosphorImager. (C) Half-life of the selAB mRNA. The signals of probe A1 obtained in three independent experiments of the type described in Figure 8B were quantified using ImageQuant 2.1 (Molecular Dynamics), normalized to an internal standard and averaged. The straight lines fitted to the linear parts of the curves by regression analysis of the data correspond to half-lives of 67 s in strain MTA2 and 52 s in strain PT90422. Download figure Download PowerPoint The SECIS-like element functions as a selenocysteine insertion element Having resolved the function of the SECIS-like structure as a novel type of regulatory element, it was tempting to assess whether it may still act as a genuine SECIS element, i.e. mediate the decoding of UGA with selenocysteine. To follow this possibility, a plasmid (pWA) was constructed that carries a lacZ gene with an in-frame UGA codon followed by the sequence encoding the SECIS-like element inserted into its 5′ region (Figure 9A). The distance between the UGA codon and the sequence of the SECIS-like element corresponding to the minimal binding region of SECIS elements was adjusted to 11 bp, which has been shown to be optimal for SelB activity (Heider et al., 1992; Chen et al., 1993; Liu et al., 1998). As a control, plasmid pWT was employed, which contains the fdhF SECIS in exactly the same position. The two plasmids were transferred into E.coli PT90422 (wild type) and MTA2 (ΔselC), and β-galactosidase activities were determined as a measure for read-through at the UGA codon (Figure 9B). The results clearly show that the SECIS-like element is able to act as an insertion sequence, but only at a reduced efficiency. Figure 9.Ability of the SECIS-like element to mediate SelB-dependent UGA read-through. (A) Schematic representation of the insert carried by plasmid pWA. The sequence of the insert replacing the fdhF SECIS cartridge of pWT is depicted as RNA in the predicted stem–loop conformation. Residues identical to those of the SECIS-like element are represented in upper case (B) β-galactosidase activities measured for strains PT90422 (WT) and MTA2 (ΔselC) transformed with pWT (fdhF SECIS) and pWA (SECIS-like element). Download figure Download PowerPoint Figure 10.Proposed model of the reactions involved in the regulation of selAB expression. Without the quaternary complex assembled at the SECIS-like element (state I), translation of selA and selB is derepressed. Biosynthesis of Sec-tRNASec (a), which is dependent on a sufficient supply of selenide, enables the formation of the regulatory complex on the 5′ mRNA structure of the selAB transcript (b), thereby decreasing the rate of translation of selA and, to a lesser extent, selB (state II). Competition between the SECIS-like element and the SECIS element of selenoprotein mRNAs (c) leads to the dissociation of the regulatory complex and to the formation of the SelB·GTP·Sec- tRNASec·SECIS complex, which catalyzes the decoding of UGA as selenocysteine. After A-site interaction and translocation of the ribosome (d), SelB and tRNASec are released and ready to re-enter the cycle. Download figure Download PowerPoint Discussion Selenocysteine insertion sequences in bacterial selenoprotein mRNAs guide the ribosome to translate in-frame UGA codons with selenocysteine. They do so by the formation of a quaternary complex with translation factor SelB, Sec-tRNASec and GTP. Concomitant binding of the SECIS element and the charged tRNASec conf