14-3-3 Proteins Regulate a Cell-Intrinsic Switch from Sonic Hedgehog-Mediated Commissural Axon Attraction to Repulsion after Midline Crossing
2012; Cell Press; Volume: 76; Issue: 4 Linguagem: Inglês
10.1016/j.neuron.2012.09.017
ISSN1097-4199
AutoresPatricia T. Yam, Christopher B. Kent, Steves Morin, W. Todd Farmer, Ricardo Alchini, Léa Lepelletier, David Colman, Marc Tessier‐Lavigne, Alyson E. Fournier, Frédéric Charron,
Tópico(s)Nerve injury and regeneration
ResumoAxons must switch responsiveness to guidance cues during development for correct pathfinding. Sonic Hedgehog (Shh) attracts spinal cord commissural axons ventrally toward the floorplate. We show that after crossing the floorplate, commissural axons switch their response to Shh from attraction to repulsion, so that they are repelled anteriorly by a posterior-high/anterior-low Shh gradient along the longitudinal axis. This switch is recapitulated in vitro with dissociated commissural neurons as they age, indicating that the switch is intrinsic and time dependent. 14-3-3 protein inhibition converted Shh-mediated repulsion of aged dissociated neurons to attraction and prevented the correct anterior turn of postcrossing commissural axons in vivo, an effect mediated through PKA. Conversely, overexpression of 14-3-3 proteins was sufficient to drive the switch from Shh-mediated attraction to repulsion both in vitro and in vivo. Therefore, we identify a 14-3-3 protein-dependent mechanism for a cell-intrinsic temporal switch in the polarity of axon turning responses.
Referência(s)