Specialized transduction of tryptophan markers in Escherichia coli K12 by bacteriophage ∅80
1963; Elsevier BV; Volume: 19; Issue: 4 Linguagem: Inglês
10.1016/0042-6822(63)90041-2
ISSN1096-0341
Autores Tópico(s)DNA and Nucleic Acid Chemistry
ResumoA UV-inducible temperate phage ∅80, capable of lysogenizing on E. coli K12, has been isolated. Some of its properties have been investigated. Prophage ∅80 is closely linked to the try marker on the chromosome of strain K12. Specialized transduction of try+ genes from tryptophan positive (try+) to tryptophan negative cells (try−) by ∅80 has been demonstrated. No markers except those in the try region have been transduced by this phage. The frequency of transduction resembles that of other transduction systems such as λ. Some try+ transductants have been found to be heterogenotes. Heterogenotic cultures produce high frequency transducing (HFT) lysates, which contain both active phage ∅80 and defective transducing phage ∅80 dt carrying try genes. Each ∅80 dt strain carries the set of genes of the tryptophan operon, replacing a part of the phage genome which includes the immunity-related locus.
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