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On the structure of liver ribosomes

1974; Elsevier BV; Volume: 86; Issue: 3 Linguagem: Inglês

10.1016/0022-2836(74)90181-8

1089-8638

N.A. Kiselev, V.Ya. Stel'mashchuk, M.I. Lerman, O. Yu. Abakumova,

Advanced Electron Microscopy Techniques and Applications

Abstract The morphology of liver ribosomes and their subparticles, large and small, has been investigated. Analysis of the images has been carried out by successive selection of models and by X-raying them under conditions simulating negative staining. The relation between the main views has been checked by tilting the specimens in an electron microscope through ± 30 °. The small subparticle consists of an elongated body, to one of the ends of which a short “head” is attached. A model has been proposed, whose projections on rotation with respect to the longitudinal axis would satisfy all observable types of images. According to the proposed model, the “head” is tilted with respect to the elongated portion. The length of the subparticle is 230 A. The dimensions of the elongated portion in the transverse direction are 110 A × 75 to 80 A. The large subparticles in lateral view resemble short “rods” 220 to 240 A long and about 70 to 95 A wide. At a distance of about 60 A from the left end of the particles a projection (60 A in length) is seen, on the inner side of which a depression, or “channel”, filled with the contrasting substance is always observed. Next to this depression a second projection is located, whose height is about 30 A. The channel is either a cavity in the body of the large subparticle or a part of the RNA without protein. The length of the channel is about 80 A, the width is about 50 to 60 A. The left end of the particles is characterized by two sharpened portions; as a result, a cavity that shows an obtuse angle profile makes its appearance. The opposite end of the particles is cut off at an angle of 45 °. In another view, the subparticles appear to be almost rectangular in shape; they are characterized by dimensions of 150 A × 220 to 240 A. It is likely that the large projection is displaced sideways with respect to the longitudinal axis of the particles. The asymmetry associated with this displacement gives rise to preferred arrangements of the subparticles on the supporting film. An analysis has been made of the most typical images of monomeric ribosomes, on the basis of which a suggestion is made about mutual orientation of subparticles in a monomer.