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Computational Analysis of Two Species C Human Adenoviruses Provides Evidence of a Novel Virus

2011; American Society for Microbiology; Volume: 49; Issue: 10 Linguagem: Inglês

10.1128/jcm.00156-11

1098-660X

Michael P. Walsh, Jason Seto, Elizabeth B. Liu, Shoaleh Dehghan, Nolan Ryan Hudson, Alexander N. Lukashev, Olga E. Ivanova, James Chodosh, David W. Dyer, Morris S. Jones, Donald Seto,

CRISPR and Genetic Engineering

Human adenovirus C (HAdV-C) species are a common cause of respiratory infections and can occasionally produce severe clinical manifestations. A deeper understanding of the variation and evolution in species HAdV-C is especially important since these viruses, including HAdV-C6, are used as gene delivery vectors for human gene therapy and in other biotechnological applications. Here, the full-genome analysis of the prototype HAdV-C6 and a recently identified virus provisionally termed HAdV-C57 are reported. Although the genomes of all species HAdV-C members are very similar to each other, the E3 region, hexon and fiber (ten proteins total) present a wide range of identity values at the amino acid level. Studies of these viruses in comparison to the other three HAdV-C prototypes (1, 2, and 5) comprise a comprehensive analysis of the diversity and conservation within HAdV-C species. HAdV-C6 contains a recombination event within the constant region of the hexon gene. HAdV-C57 is a recombinant virus with a fiber gene nearly identical to HAdV-C6 and a unique hexon distinguished by its loop 2 motif.