Quantification and Facilitated Comparison of von Willebrand Factor Multimer Patterns by Densitometry
2001; Oxford University Press; Volume: 116; Issue: 4 Linguagem: Inglês
10.1309/75cq-v7ux-4qx8-wxe7
ISSN1943-7722
AutoresJ.-D. Studt, Ulrich Budde, Reinhard Schneppenheim, R. Eisert, M. von Depka Prondzinski, Arnold Ganser, M. Barthels,
Tópico(s)Complement system in diseases
ResumoThe analysis of von Willebrand factor (vWF) multimers is an important laboratory tool for distinguishing among the numerous subtypes of von Willebrand disease (vWD). Comparability and reproducibility of this method are insufficient; standardization and external references are pending. Interlaboratory comparison of results therefore may be difficult. We applied densitometry to obtain a reproducible quantification of vWF multimer patterns in healthy donors, patients with vWD variants, and factor VIII/vWF concentrates to improve the reproducibility and comparability of vWF multimer analysis. Multimers were separated and visualized luminographically on x-ray films. Films were scanned and evaluated by densitometry. The variation inherent in vWF multimer analysis and the range of the normal could be quantified. In vWD variants and factor VIII/vWF concentrates, densitometry could quantify and visualize alterations of vWF multimer patterns and facilitate their comparison. Densitometry permits a precise quantitative comparison of sample patterns to a reference plasma. It could be a valuable tool offering reproducible quantification and additional visualization of normal and pathologic vWF multimer patterns, facilitating their comparison and contributing to a standardization of vWF multimer analysis.
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