Cytokine Expression by First‐Trimester Human Chorionic Villi
1998; Wiley; Volume: 40; Issue: 5 Linguagem: Inglês
10.1111/j.1600-0897.1998.tb00059.x
ISSN1600-0897
AutoresWilliam A. Bennett, Sandhya Lagoo‐Deenadayalan, J.A. Stopple, William H. Barber, Enatra Hale, M.N. Brackin, Bryan D. Cowan,
Tópico(s)Reproductive Physiology in Livestock
ResumoPROBLEM: Communication at the human maternal‐fetal interface occurs by an intricate cytokine network. This study examines cytokine expression by normal first‐trimester human chorionic villi. METHOD OF STUDY: Tissues were obtained at elective pregnancy terminations (7–9 weeks). Total RNA was isolated from chorionic villi by guanidinium isothiocynate‐acid phenol extraction. A reverse transcriptase‐polymerase chain reaction technique was used to examine cytokine expression. β‐Actin was used as the housekeeping gene, and mitogen‐stimulated lymphocytes served as positive controls. RESULTS: β‐Actin was uniformly expressed by all chorionic villous samples. Interferon (IFN)‐α and ‐β also were highly expressed. Moderate expression was noted for interleukin (IL)‐10, IL‐6, tumor necrosis factor (TNF)‐α, and IL‐1β. In contrast, transforming growth factor‐β1, IFN‐γ, IL‐2, and IL‐1α were either weakly expressed or absent in first‐trimester villi. CONCLUSIONS: Cytokines may contribute to pregnancy immunotolerance (IFN‐α, IFN‐β, and IL‐10), viral resistance (IFNs), hormone secretion (IL‐1 and IL‐6), and cellular remodeling (IFN‐γ and TNF‐α) within the chorionic villous.
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