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Receptor for pre‐Sl(21–47) component of hepatitis B virus on the liver cell: Role in virus cell interaction

1992; Wiley; Volume: 37; Issue: 2 Linguagem: Inglês

10.1002/jmv.1890370208

1096-9071

Srikant Dash, Kanury V. S. Rao, Subrat Kumar Panda,

Liver Disease Diagnosis and Treatment

Abstract Attachment of hepatitis B virus to a hepatoblastoma cell line (HepG2) was examined using a synthetic peptide corresponding to the pre‐S1 (21‐47) region of the envelope protein. Scatchard analysis revealed a single class binding site of Kd 104 ± 27 nM/I and 5.4 ± 1.2 × 10 5 sites per cell. Competition of HBV with pre‐S1 peptides was dose dependent, and demonstrated it as the dominant binding site. In view of the suggested sequence homology between the peptide and IgA, cross‐competition studies were carried out. The results indicate no direct role of IgA receptor in HBV binding. The receptor for the pre‐S1 pep‐ tide was identified as a single major peptide of molecular weight 31 kD using in‐situ ligand receptor crosslinking. © 1992 Wiley‐Liss, Inc.