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BIBTEX RIS

The akuB KU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus

2006; American Society for Microbiology; Volume: 5; Issue: 1 Linguagem: Inglês

10.1128/ec.5.1.207-211.2006

ISSN

1535-9778

Autores

Márcia Eliana da Silva Ferreira, Márcia Regina von Zeska Kress, Marcela Savoldi, Maria Helena S. Goldman, Albert Härtl, Thorsten Heinekamp, Axel A. Brakhage, Gustavo H. Goldman,

Tópico(s)

Fungal and yeast genetics research

Resumo

ABSTRACT To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuB KU80 ). Homologous integration reached about 80% for both calcineurin A ( calA ) and polyketide synthase pksP (alb1 ) genes in the akuB KU80 mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB KU80 had no influence on pathogenicity in a low-dose murine infection model.

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