Produção Nacional
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni
2008; Baishideng Publishing Group; Volume: 14; Issue: 38 Linguagem: Inglês
10.3748/wjg.14.5842
ISSN2219-2840
AutoresSheilla Andrade de Oliveira, Bruno Solano de Freitas Souza, Carla Adriana Guimarães-Ferreira, Elton Sá Barreto, Siane Campos de Souza, Luiz Antônio Rodrigues de Freitas, Ricardo Ribeiro‐dos‐Santos, Milena Botelho Pereira Soares,
Tópico(s)Clinical Nutrition and Gastroenterology
ResumoAIM: To investigate the potential of bone marrow mononuclear cells (BM-MCs) in the regeneration of hepatic lesions induced by Schistosoma mansoni (S.mansoni ) chronic infection.METHODS: Female mice chronically infected with S.mansoni were treated with BM-MCs obtained from male green fluorescent protein (GFP) transgenic mice by intravenous or intralobular injections.Control mice received injections of saline in similar conditions.Enzyme-linked immunosorbent assay (ELISA) assay for transforming growth factor-beta (TGF-β), polymerase chain reaction (PCR) for GFP DNA, immunofluorescence and morphometric studies were performed.RESULTS: Transplanted GFP + cells migrated to granuloma areas and reduced the percentage of liver fibrosis.The presence of donor-derived cells was confirmed by Fluorescence in situ hybridization (FISH) analysis for detection of cells bearing Y chromosome and by PCR analysis for detection of GFP DNA.The levels of TGF-β, a cytokine associated with fibrosis deposition, in liver fragments of mice submitted to therapy were reduced.The number of oval cells in liver sections of S.mansoni -infected mice increased 3-4 fold after transplantation.A partial recovery in albumin expression, which is decreased upon infection with S.mansoni , was found in livers of infected mice after cellular therapy.CONCLUSION: In conclusion, transplanted BMCs migrate to and reduce the damage of chronic fibrotic liver lesions caused by S.mansoni .
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