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In vivo survival rate of rabbit morulae after vitrification in a medium without serum protein

1999; Elsevier BV; Volume: 39; Issue: 5-6 Linguagem: Inglês

10.1051/rnd

1297-9708

Jose-Salvator Vicente, M.P. Viudes-de-Castro, M. L. García,

Sperm and Testicular Function

The in vivo survival rate of rabbit morulae after vitrification in a mixture of dimethyl sulphoxide and ethylene glycol solution without protein supplement (WPS) was compared with two types of protein supplements: rabbit serum (RS) and bovine serum albumin (BSA).Significant dif- ferences were observed in the percentage of transferable embryos (undamaged embryos after devit- rification, 80.4 % versus 93.2 and 92.1 %, WPS, BSA and RS, respectively, P < 0.05) and live born rate (40.9 % versus 56.1 %, WPS and BSA, respectively, P < 0.05).Non-significant differences were, however, observed in the percentages of implanted embryos at 12 days post-ovulation induc- tion (56.7, 69.7 and 68.6 %), post-implantation survival rate (82.3, 74.2 and 77.2 %) and live born rate in pregnant does (54.6, 56.1 and 50.5 %) with different vitrification media (RS, BSA and WPS).We conclude that rabbit embryos can be vitrified and stored using protein-free vitrification medium with moderate losses of viability.© Inra/Elsevier, Paris rabbit embryos / vitrification / protein-free medium