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BIBTEX RIS

In Vivo Protein-Protein Interaction Assays: Beyond Proteins

2001; Wiley; Volume: 40; Issue: 5 Linguagem: Inglês

10.1002/1521-3773(20010302)40

ISSN

1521-3773

Autores

Hening Lin, Virginia W. Cornish,

Tópico(s)

Fungal and yeast genetics research

Resumo

The scope of cell-based assays is being expanded to allow the detection of interactions between proteins and DNA, RNA, or small molecules. The yeast two-hybrid assay, which normally detects protein–protein interactions, can be modified to detect interactions between ligands (the two dark yellow shapes in the diagram) and receptors (DHFR or GR). A small bridging molecule (made up of the ligands joined together) is used to join two fusion proteins (DHFR–DBD and AD–GR), thereby activating the reporter gene.

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