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New method for HPLC separation and fluorescence detection of malonaldehyde in normal human plasma

2006; Elsevier BV; Volume: 832; Issue: 1 Linguagem: Inglês

10.1016/j.jchromb.2005.12.041

1873-376X

Jianjun Mao, Hongwu Zhang, Jia Luo, Li Li, Rui Zhao, Renen Zhang, Guoquan Liu,

Electrochemical sensors and biosensors

A new method for the detection of free and total malonaldehyde (MDA) in human plasma samples based on the derivatization of MDA with 9-fluorenylmethoxycarbonyl hydrazine (FMOC-hydrazine) in an acidic medium was developed. Derivatization was achieved after 4 h at 50 °C. The derivatized samples were analyzed by HPLC using a reversed-phase C18 column with fluorescence detection (Ex = 270 nm, Em = 310 nm). The benefit of this direct injection of deproteinized plasma is to avoid the use of an internal standard. The detection limit was 0.1 pmol (4.0 nmol/L). The recovery of MDA spiked in different human plasma samples was 95.3% (n = 25; R.S.D. 5.1%) for the hydrolysation procedure. The total and free MDA in plasma of 15 healthy male volunteers are 426 ± 29.8 nmol/L and 153 ± 9.6 nmol/L, respectively.