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Molecular Characterization of a Novel Botulinum Neurotoxin Type H Gene

2013; Oxford University Press; Volume: 209; Issue: 2 Linguagem: Inglês

10.1093/infdis/jit450

1537-6613

Nir Dover, Jason R. Barash, Karen K. Hill, Gary Xie, Stephen S. Arnon,

Neurological disorders and treatments

We sequenced the 2 botulinum toxin gene clusters of Clostridium botulinum strain IBCA10-7060 type Bh. The sequence of bont/H differed substantially from the sequences of the 7 known bont genes for toxin types A–G. The 5′ one-third terminus of bont/H that codes for the botulinum toxin light chain differed markedly from the light chain coding sequences of toxin types A–G. The 3′ two-thirds terminus of bont/H that codes for the botulinum toxin heavy chain contained a novel Hn translocation domain coding sequence and a nonneutralizing type A–like Hc binding domain coding sequence. bont/H was part of an orfX toxin gene cluster that was located at a unique chromosomal site distant from those used by other botulinum toxin gene clusters. The bont/B sequence was similar to that of subtype bont/B2 and was located within its ha toxin gene cluster at the oppA/brnQ site. Our findings further establish that C. botulinum IBCA10-7060 produces novel BoNT/H.