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Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping

1991; Oxford University Press; Volume: 19; Issue: 2 Linguagem: Inglês

10.1093/nar/19.2.303

1362-4962

John Welsh, Charles E. Petersen, Michael McClelland,

RNA Research and Splicing

Polymorphisms in genomic fingerprints generated by arbitrarily primed PCR (AP-PCR) can distinguish between strains of almost any organism. We applied the technique to the mouse (Mus musculus) . The characteristic differences In the AP-PCR genomic fingerprints between strains will be of value in strain identification and verification. Using one primer, we genetically mapped four polymorphisms in a set of C57BL/6J × DBA/2J recombinant Inbreds. One of these polymorphisms is a length variant. The method will allow rapid genetic mapping of DNA polymorphisms without Southern blotting.