Portal de Periódicos da CAPES

Effects of spaceflight on innate immune function and antioxidant gene expression

2009; American Physiological Society; Volume: 106; Issue: 6 Linguagem: Inglês

10.1152/japplphysiol.91361.2008

8750-7587

Farnaz P. Baqai, Daila S. Gridley, James M. Slater, Xian Luo‐Owen, Louis Stodieck, Virginia L. Ferguson, Stephen K. Chapes, Michael J. Pecaut,

High Altitude and Hypoxia

Spaceflight conditions have a significant impact on a number of physiological functions due to psychological stress, radiation, and reduced gravity. To explore the effect of the flight environment on immunity, C57BL/6NTac mice were flown on a 13-day space shuttle mission (STS-118). In response to flight, animals had a reduction in liver, spleen, and thymus masses compared with ground (GRD) controls (P < 0.005). Splenic lymphocyte, monocyte/macrophage, and granulocyte counts were significantly reduced in the flight (FLT) mice (P < 0.05). Although spontaneous blastogenesis of splenocytes in FLT mice was increased, response to lipopolysaccharide (LPS), a B-cell mitogen derived from Escherichia coli, was decreased compared with GRD mice (P < 0.05). Secretion of IL-6 and IL-10, but not TNF-alpha, by LPS-stimulated splenocytes was increased in FLT mice (P < 0.05). Finally, many of the genes responsible for scavenging reactive oxygen species were upregulated after flight. These data indicate that exposure to the spaceflight environment can increase anti-inflammatory mechanisms and change the ex vivo response to LPS, a bacterial product associated with septic shock and a prominent Th1 response.