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Mycobacterium tuberculosis nitrogen assimilation and host colonization require aspartate

2013; Nature Portfolio; Volume: 9; Issue: 11 Linguagem: Inglês

10.1038/nchembio.1355

1552-4469

Alexandre Gouzy, Gérald Larrouy-Maumus, Ting‐Di Wu, António Peixoto, Florence Levillain, Geanncarlo Lugo‐Villarino, Jean‐Luc Guerquin‐Kern, Luiz Pedro S. de Carvalho, Yannick Poquet, Olivier Neyrolles,

Diagnosis and treatment of tuberculosis

Aspartate is a primary nitrogen source for Mycobacterium tuberculosis during infection, being required for virulence after entering cells via the amino acid transporter AnsP1. Here we identify the amino acid transporter AnsP1 as the unique aspartate importer in the human pathogen Mycobacterium tuberculosis. Metabolomic analysis of a mutant with an inactive AnsP1 revealed that the transporter is essential for M. tuberculosis to assimilate nitrogen from aspartate. Virulence of the AnsP1 mutant is impaired in vivo, revealing that aspartate is a primary nitrogen source required for host colonization by the tuberculosis bacillus.