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Sex determination by CHDW and CHDZ genes of avian sex chromosomes in Nymphicus hollandicus

2007; Scientific and Technological Research Council of Turkey (TUBITAK); Volume: 31; Issue: 6 Linguagem: Inglês

1303-6181

Harun Cerit, Kozet Avanus,

Bird parasitology and diseases

The aim of this study was sex determination in Nymphicus hollandicus without giving it any harm and obtaining accurate results by DNA analysis. CHD genes are preserved within avian Z and W sex chromosomes. The intron regions of the CHDW and CHDZ genes vary between male (ZZ) and female (ZW) individuals. The method used in this study was based on this difference. DNA was extracted from feathers instead of blood. The intron regions of CHDW and CHDZ genes were amplified by sex specific primers (P2 and P8). PCR products were screened by agarose gel electrophoresis. Individuals showing double (ZW) and single (ZZ) bands were identified as females and males, respectively. Ozet: Bu cal›flman›n amac›, Nymphicus hollandicus'lara (sultan papaan›) fiziksel bir zarar vermeden DNA analiz metoduyla cinsiyet saptamas› yapmakt›r. CHD genleri kanatl› Z ve W cinsiyet kromozomlar› aras›nda korunmufltur. CHD geninin intron bolgesi erkek (ZZ) ve difli (ZW) bireyler aras›nda farkl›l›k gostermektedir. Cal›flmada kullan›lan metotta bu farkl›l›k esas al›nm›flt›r. DNA kan yerine tuyden izole edilmifltir. PCR uygulamas› CHDW ve CHDZ genlerinin intron bolgesini coaltan cinsiyete ozel primerler kullan›larak (P2 ve P8) gerceklefltirilmifltir. PCR urunleri agaroz jel elektroforezi ile goruntulenmifltir. Sonuc olarak, cift bant gosterenlerin difli, tek bant gosterenlerin erkek olduu saptanm›flt›r. Anahtar Sozcukler: Nymphicus hollandicus, CHD1W, CHD1Z, kanatl›, cinsiyet tayini